Grans can be separated out using Ficoll-Hypaque with 1.088 specific gravity.
The PBS diluted blood would be layered over the ficoll-hypaque and
centrifuged at 250g (at the buffer F-H interface) for 20-25 minutes..
Sigma used to carry that density, but I don't know whether they do any more.
Marj Williams
Lehigh Valley Hospital
Allentown, PA
610-402-8016
Subject: gradient separation for neutrophil rich plasma
Date: Mon, 6 Feb 2006 20:42:15 -0000
From: Nebe-Von-Caron.G[g.nebe-von-caron]@unipath.com
To: cyto-inbox
X-HDT-HopCount: 1
I try to remember a simple gravity sedimentation method for blood to get
granulocyte rich white blood cell reparations. It worked by just
layering I think PBS diluted blood over a density gradient and allowing
the blood to settle whilst standing on the bench. As there was no
pre-mixing with dextran it minimised activation. Unfortunately I did not
keep any records about it but I . Anyone got any comments or memories of
it? I might have even discussed it over the Purdue server but could not
find any record.
Thanks in advance
Gerhard
Message
I try
to remember a simple gravity sedimentation method for blood to get granulocyte
rich white blood cell reparations. It worked by just layering I think PBS
diluted blood over a density gradient and allowing the blood to settle whilst
standing on the bench. As there was no pre-mixing with dextran it minimised
activation. Unfortunately I did not keep any records about it but I . Anyone got
any comments or memories of it? I might have even discussed it over the Purdue
server but could not find any record.
Thanks
in advance
Gerhard
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Received on Wed Feb 8 16:35:07 2006
