RE: annexin V and RBC lysing

   Hi Indresh,
    As I recall, annexin binds to platelets, so trying to label whole blood
may be problematic.
regards,
Tom
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* 
Thomas W. Mc Closkey, Ph. D. 
Scientist in Charge, Flow Cytometry 
The Institute for Medical Research, North Shore University Hospital 
Assistant Professor of Pediatrics, New York University School of Medicine 
Biomedical Research Center, 350 Community Drive 
Manhasset, Long Island, New York 11030 
ph:  516-562-4844 [office], 516-562-1084 [lab] 
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* 




   _____  

From: IKaur@mdanderson.org [mailto:IKaur@mdanderson.org] 
Sent: Tuesday, January 31, 2006 3:37 PM
To: cyto-inbox
Subject: annexin V and RBC lysing



We are staining cells for AnnexinV and PI (using the kit from Pharmingen) to
look for apoptotic cells, but also need to lyse the RBCs. Should the RBCs be
lysed before or after the AnnexinV staining. We are using Pharmlyse ammonium
chloride lysing solution.
Also, has anyone used 7AAD instead of PI. 


Indresh Kaur, Ph.D.
Coordinator, Flow Cytometry Lab
Department of Blood & Marrow Transplantation
713-563-4811 

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