Greetings from a very wet Vermont! Is anyone routinely doing Calcium flux on the LSRII? I am having a problem with setting a tight baseline when looking at ratio vs. time. It appears that there are some cells that are already in flux, when the cells first go through the flow cell. I am unsure if it has to do with the handling of the cells by the investigator, or if this is operator error. I have carefully followed the instructions provided by BD but this is a reoccurring problem. Any guidance or comments are appreciated. Colette Charland Researcher/Analyst University of Vermont 89 Beaumont Ave. Flow Cytometry Center/Given C316 Burlington, VT 05405 1-802-656-8742Received on Fri Feb 3 11:58:00 2006
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