Long ago we have used TUNEL method to measure induction of apoptosis during leukemia treatment. I believe that still it is the most reliable approach to detect apoptosis and measure its incidence in leukemias. Gorczyca, W., Bigman, K., Mittelman, A., Ahmed T., Gong J., Melamed, M.R., Darzynkiewicz, Z.: Induction of DNA strand breaks associated with apoptosis during treatment of leukemias. Leukemia, (Baltimore) 7: 659-670, 1993. Li, X., Gong, J., Feldman, E., Seiter, K., Traganos, F. Darzynkiewicz, Z.: Apoptotic cell death during treatment of leukemias. Leukemia and Lymphoma, 13: Suppl. 1, 65-70, 1994. Zbigniew Darzynkiewicz, M.D., Ph.D. Brander Cancer Research Institute at NYMC 19 Bradhurst Avenue, Suite 2400 Hawthorne, N.Y. 10532 darzynk@nymc.edu http://www.darzynkiewicz.com/zbigniew/ -----Original Message----- From: alireza [mailto:arardjmand@yahoo.com] Sent: Tuesday, January 10, 2006 12:19 AM To: cyto-inbox Subject: In vivio apoptosis detction Dear Flowers I am going to evaluate the apoptosis induction within the Lukemic cells of patients following treatment specially by Flow. in other words invivo apoptosis detection, any experences in this regard is highly appreciated. Kindest regrads, Alireza Ardjmand Hematology, Oncology and BMT research center Shariati Hospital, Kargar Ave. 14114 Tehran, Iran Tel: +98(21) 84902695 +98(21) 88029390 Home:+98(21)44252464 Fax:+98(21) 88004140 ________________________________ Yahoo! Photos Ring in the New Year with Photo Calendars <http://us.rd.yahoo.com/mail_us/taglines/photos/*http:/pa.yahoo.com/*htt p:/us.rd.y%0d%0aahoo.com/mail_us/taglines/photos/evt=38087/*http:/pg.pho tos.yahoo.com/ph/page?.file=cal%0d%0aendar_splash.html&.dir=> . Add photos, events, holidays, whatever.Received on Wed Jan 11 15:58:00 2006
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