Hi everybody, I am a new member to the cytometry mailing list and have just started my basic science research. I am working on sorting SP cells based on Hoechst analysis and would like to isolate RNA from this small cell population (10,000-20,000cells). I was isolating the cells directly in Trizol on ice but this is not giving me a good RNA integrity. Does anybody have a protocol for isolation of small cell populations and then RNA isolation from them which gives a good yield and quality of RNA. I am very new to research and hope somebody can help me with this, Thanks in advance. Gaurav Kapur Children's Hospital of Michigan Michigan email: gkapur@dmc.orgReceived on Tue Jan 10 11:58:00 2006
This archive was generated by hypermail 2.1.8 : Sat Jan 14 2006 - 22:12:27 EST
