We routinely get 10ng of RNA from 3000 sorted human CECs, and have used the RNA successfully for Affymetrix chips. Phil ________________________________ From: Alexandre Trindade [mailto:alexandret@netcabo.pt] Sent: Sunday, January 01, 2006 3:47 PM To: Cytometry Mailing List Subject: RNA content in endotelial cells Hi I'm new here and I'm really new to flow cytometry. I'm working on sorting endothelial cells from mouse embryos and then extract RNA from them. I'm already working on the cytometer trying to establish the technique but I'm trying to plan my work ahead and one thing I'm trying to find out is how much RNA, in average, can I expect to extract from a endothelial cell. I would need this figure so I can plan how many embryos do I need to achieve the amount of RNA I have been asked. Ethics department requires me to be as efficient as I can, please help me out. Thanks in advance. Regards Alexandre Trindade ps: Happy New Year!!Received on Wed Jan 4 14:38:00 2006
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