Re: RNA content in endotelial cells

Not so sure. But you might get 1x10e(-5) ug per cell,
which means you need to have 0.1 millions cells to get
1ug total RNA(with some variations). But sometimes you
still get enough total RNA from lower number of  cells
for RT-PCR.

Li 
--- Alexandre Trindade <alexandret@netcabo.pt> wrote:

> Hi
> 
> I'm new here and I'm really new to flow cytometry.
> I'm working on sorting endothelial cells from mouse
> embryos and then extract RNA from them. I'm already
> working on the cytometer trying to establish the
> technique but I'm trying to plan my work ahead and
> one thing I'm trying to find out is how much RNA, in
> average, can I expect to extract from a endothelial
> cell. I would need this figure so I can plan how
> many embryos do I need to achieve the amount of RNA
> I have been asked. Ethics department requires me to
> be as efficient as I can, please help me out.
> Thanks in advance.
> Regards
> Alexandre Trindade
> 
> ps: Happy New Year!!



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