Simona: It is probably one of two things, either your vacuum is not strong enough or your solenoid valve which controls this function is bad or stuck. I would check your vacuum line filter to make sure it does not need to be changed (the filter will be off color). It may be clogged enough to reduce your vacuum where it is strong enough for your waste but not for the nozzle flush. The vacuum pressure on the nozzle flush has to be greater than the sheath pressure. Also you might want to check your source of vacuum (house or otherwise) to make sure it is up to par. As far as the solenoid, I believe it is V3. I would first try and tap it to make sure it isn't just stuck. If that doesn't work you might have to replace it. Good Luck!! Joanne Lannigan, M.S. Director, Flow Cytometry Core University of Virginia On Fri, 16 Dec 2005 14:38:16 +0100 Simona Ronzoni <simona.ronzoni@ifom-ieo-campus.it> wrote: > Hi all, > I have a problem with Fill and nozzle flush on Facs Vantage SE + turbo >optional > I work at 20 PSI and when the nozzle is clogged I put on fill and I push >the bottom nozzle flush, but the stream go to back very slowly and is not >enough to clean the nozzle. > This happen also when the nozzle is not clogged!! > > Any comments are welcome > Many thanks in advance > > Simona > > > -- > Simona Ronzoni >Flow Cytometry and Imaging Core > Experimental Oncology Department > European Institute of Oncology > via Ripamonti, 435 20141 Milan Italy > Phone: ++39-02-57489880 Fax: ++39-02-57489851 > e-mail: simona.ronzoni@ifom-ieo-campus.it > http://www.ifom-ieo-campus.it > http://imaging.altervista.orgReceived on Mon Dec 19 14:38:00 2005
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