Re: anybody ever sort out ...

Larry
Could I've the adress who sell those small beads? Did you use stain for
invitrogen antibody monoclonal complx V mito? Should I do fixation before
permeabilisation tor flow immage?
Peni
Biophysic dept.
Medical Faculty
----- Original Message -----
From: "Larry Arnold" <lwarma@med.unc.edu>
To: cyto-inbox
Sent: Wednesday, December 07, 2005 8:54 PM
Subject: Re: anybody ever sort out ...


> Chris
>
> We are getting ready to do something similar on our MoFlo.  We
> already sort some small bacteria with no problem (SSC log
> trigger).  We looked at polystyrene beads recently and were easily
> able to resolve beads down to 0.21um diameter. If the mito stain
> bright enough you could also trigger on them.
>
> Larry
>
> At 04:23 PM 12/5/2005, you wrote:
> >mitochondria?
> >
> >
> >I've got somebody that wants to isolate mitochondria so they can
> >extract proteins.
> >
> >She says she can stain them.  But I'm just wondering about their
> >size, relative to the
> >debris we'll have.
> >
> >thanks,
> >
> >Chris
>
> Larry W. Arnold, Ph.D.
> Research Professor and Director, Flow Cytometry Facility
> Department of Microbiology and Immunology
> CB# 7290
> University of North Carolina
> Chapel Hill, NC 27599
> Phone: 919-966-1530
> FAX: 919-962-8103
>
>