Re: anybody ever sort out ...[mitochondria]

Chris Worth wrote:

>mitochondria?
>
>
>I've got somebody that wants to isolate mitochondria so they can 
>extract proteins.
>
>She says she can stain them.  But I'm just wondering about their 
>size, relative to the
>debris we'll have.


FCM of isolated mitochondria dates back to O'Connor et al, 1988, one 
of many references in two good recent papers on the subject in Cytometry:


Mattiasson G:Flow cytometric analysis of isolated liver mitochondria 
to detect changes
relevant to cell death. Cytometry A. 2004; 60:145-54.

Mattiasson G: Analysis of mitochondrial generation and release of 
reactive oxygen species.
Cytometry A. 2004 Dec;62(2):89-96.

-Howard