Hi John: I'm not sure quite what you mean by "establishing the DNA content model"? What species are you analyzing? We use both flow cytometry and PI routinely for plant nuclear DNA content and ploidy measurements. Please send me more details and we'll try to troubleshoot your problem. Best wishes David Galbraith On Tue, 8 Nov 2005 jtigges@caregroup.harvard.edu wrote: > Hello All, I was wondering if anyone had any insight on staining plant > nuclei with PI. We have a researcher who is staining plant nuclei using > Otto I and then Otto II buffer with seperate PI(2 step procedure). We > have been having problems establishing the DNA content model. Thanks in > Advance, > > John Tigges > > Core Facility Manager > Flow Cytometry > Beth Israel Deaconess Medical Center > 330 Brookline Avenue > Boston, MA 02215 > (617) 667-4901 > (617) 975-5536 > > ________________________________ > >Received on Thu Nov 10 12:18:00 2005
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