(MoFlo) Counters

Janet, Frank, James,

In my opinion this issue relates to any sorter. As far as my  
experience goes (from MoFlo, predominantly) counters are always  
right, and higher sort rates do not affect their indications. We  
routinely sort between 25,000 and 45,000 per second, and verify our  
sorts by the following approach, that you can easily also try :

 From the submitted sample, at the same speed, you sort a known  
number of cells, and re-analyze the collection tube after addition of  
a known quantity of counting beads. This will show you that your  
sorts produced the expected number of events (within let's say 5%).  
At the same time, it will allow you to quickly verify sort purity.

I now always "claim" a certain proportion of all samples for this  
simple test. It avoids unjustified "warranty claims" ...

Jan




Jan Bayer, Ph.D.
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