RE: 200um tip on moflo

From: McCoy, J. Philip (NIH/NHLBI) <mccoyj@NHLBI.NIH.GOV> Date: Fri Oct 14 2005 - 16:23:04 EST · This archive was generated by hypermail 2.1.8 : Sat Jan 14 2006 - 22:03:56 EST

Yes, we sorted C elegans worms using a 200u tip on the MoFlo. Go very low on
pressure (6psi or so,..the gauge is not accurate at these pressures), high
amplitude (nearly peaked), and low frequency (<10,000). 

Phil

-----Original Message-----
From: James Marvin [mailto:j-marvin@northwestern.edu] 
Sent: Friday, October 14, 2005 1:37 PM
To: cyto-inbox
Subject: 200um tip on moflo

Hello
Has anybody gotten the 200um tip to work on the moflo. I seem to remember it
being pretty tough to get a good breakoff and stable stream with the 120.
Freq? Amp? Pressure?

Thanks for all your help
Marv