Hi Dr. Mangada, I think lower temp. may help to slow down the internalization of surface receptor. I have also tried using sodium azide at 0.1% which may achieve similar results. However, I have not compared the two methods side by side. Regards, Albert -----Original Message----- From: Maloy Mangada [mailto:Maloy.Mangada@acambis.com] Sent: Thursday, September 29, 2005 5:12 AM To: cyto-inbox Subject: On ice or room temperature? Hi All, Just wondered if there is a consensus on whether cell surface staining (or intracellular staining) is better at 4 deg C (on ice) or room temperature. I've done all of my staining at 4 deg (per recommendation of the Ab manufacturer) with good results but I've scanned the recent literature and it seems that all methods papers I've looked at stains at RT. Looking forward to your comments before amending my procedure! Maloy Mangada, PhD Scientist, Cell Biology Acambis, Inc., 38 Sidney St., Cambridge, MA 02139 Email: maloy.mangada@acambis.com Phone: (617) 761-4288 FAX: (617) 494-1741 _____ This e-mail is intended for the exclusive use of the addressee(s) and may contain information that is confidential, privileged, and exempt from disclosure under applicable law. If you are not the intended recipient, be advised you have received this message in error and that any use, dissemination, forwarding, printing, or copying is strictly prohibited. If you received this email by mistake, please notify the sender immediately and destroy all copies of this message and any attachments. Please visit the website at <http://www.acambis.com/> www.acambis.comReceived on Fri Sep 30 13:58:00 2005
This archive was generated by hypermail 2.1.8 : Sat Jan 14 2006 - 22:03:55 EST
