Human platelets for FACS analysis

	Dear Flowers,

	I just start working with human platelets and have no prior experience with it.  In my first experiment looking at rTK on platelets, I was not sure if I used the correct FSC and SSC.  I set both FSC and SSC in log scale and the gain was E02.  I saw a large, square population at lower right corner (assembly platelets) and a small population at upper right corner (rbc ?)	I also tried lowering the gain to E01 as suggested in the archive but did not see a diagonal center population.  Any insight on the parameter for looking at human platelets would be appreciated.

	The rTK Ab we used was recommended at conc. 2ug/ml for 1e9 platelets and it seems low to me but your expertise would be helpful in terms of a general rule of thumb for primary Ab to platelet ratio.

	I would also appreciate any recommendation on reviews or publications on the topic.

	Thanks very much in advance for your help.

	Best regards,

	Albert Tai
	Exelixis Inc.