Date: Wed Aug 24 2005 - 14:48:51 EST
I am trying to determine migration of rates of DC to DLN by flow in a porcine model. The mechanical method I have been using, grinding over a mesh screen, has been disrupting the DC. I need a method, mechanical or enzymatic, that will allow complete dissociation of the node into a single cell suspension without disrupting or stripping the cell surface markers of the DC allowing me to quantitate the number of DC and the number of lymphocytes. I am targeting the inguinal nodes of the pig. Regards, Cheryl Dean (Embedded image moved to file: pic05628.gif) Cheryl Dean Scientist / Advanced Drug Delivery BD Technologies 21 Davis Drive, Research Triangle Park, NC 27709 USA tel: (919) 597-6379 fax: (919) 597-6400 E-mail: cheryl_h_dean@bd.com Website: www.bd.com ----------------------------------------- ************************************************** IMPORTANT MESSAGE FOR RECIPIENTS IN THE U.S.A: This message may constitute an advertisement of BD group's products or services or a solicitation of interest in them. If this is such a message and you would like to opt out of receiving future advertisements or solicitations from this BD group, please forward this e-mail to the optoutbygroup@bd.com. ************************************************** This message (which includes any attachments) is intended only for the designated recipient(s). It may contain confidential or proprietary information and may be subject to attorney-client privilege or other confidentiality protections. If you are not a designated recipient, you may not review, use, copy or distribute this message. If you receive this is in error, please notify the sender by reply e-mail and delete this message. Thank You ************************************************** Corporate Headquarters Mailing Address: BD (Becton, Dickinson and Company) 1 Becton Drive Franklin Lakes, NJ 07417 U.S.A.
