Hi Dennis: We have no problem detecting (or sorting) DiI-LDL using a 488nm laser at 100mw. I have not looked at BODIPY in comparison. I have attached a data plot so you can see the intensity. No need to worry about losing your 514. Joanne Lannigan, MS Director, Flow Cytometry Core Facility University of Virginia Jordan Hall, Room 7067 P.O. Box 800734 Charlottesville, VA 22908-0734 Office: 434-924-0274 Lab: 434-243-2695 Fax: 434-982-1071 email: joannelannigan@virginia.edu > -----Original Message----- > From: Dennis J. Young [mailto:djyoung@ucsd.edu] > Sent: Tuesday, August 16, 2005 2:09 PM > To: Cytometry Mailing List > Subject: LDL > > As we are looking forward to ending the use of our water-cooled 300C (and > thereby losing the 514 nm laser line, among others): > > Has anyone compared BODIPY-LDL (488 nm) to Dil-LDL (514 nm) labeling when > used for fibroblast/smooth muscle sorting? > That is, not to double label, but to replace the 514 excitation with 488. > > > ---Dennis > > Dennis J. Young > Flow Cytometry Core Facility > Old Address: > University of California, San Diego > Bldg #4, Room 126 > 9500 Gilman Drive > La Jolla CA 92093-0671 > > Future Address (Relocation on Hold): > Moores UCSD Cancer Center > 3855 Health Sciences Drive #0815 > La Jolla, CA 92093-0815 > > Mail:<<mailto:djyoung@ucsd.edu>> > WWW:<<http://cancer.ucsd.edu/flow/>> > Telephone:(858) 822-0407 FAX: (858) 822-0403
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