Date: Fri Aug 12 2005 - 11:13:34 EST
Guy: I do not know what it means to everyone else but to us here at UVa it means the channel which is used for staining everything you do not want, i.e. an exclusion channel. For instance, if you want to exclude CD4, CD8, NKs, and CD11C and CD11b and dead cells, you would use a viability dye and a fluorochrome for your antibodies that would all fall in the same channel. What most people do is have their "dump" antibodies biotinylated so they can just add SA of whatever color they want to make their dump channel for flexibility. The sample is then gated on the negative cells in this channel, "dumping" all the positively stained cells. It works really well for cleaning up very heterogeneous cell populations. It is a critical component of doing tetramer or other rare event analysis/sorting. Hope that helps- Joanne Lannigan, MS Director, Flow Cytometry Core Facility University of Virginia Jordan Hall, Room 7067 P.O. Box 800734 Charlottesville, VA 22908-0734 Office: 434-924-0274 Lab: 434-243-2695 Fax: 434-982-1071 email: joannelannigan@virginia.edu -----Original Message----- From: Guy Hermans [mailto:Guy.Hermans@ablynx.com] Sent: Thursday, August 11, 2005 2:17 AM To: cyto-inbox Subject: What is a dump channel? Hi all, After doing quite some reading on the list and in publications, I got the impression various people use the term "dump channel" in reference to different concepts/uses. Could some of the gurus out there clarify this one for me and the rest of us out there? thanks, Guy <http://www.ablynx.com/> Next generation therapeutic antibodies Guy Hermans, PhD Project Leader Ablynx NV Technologiepark 4 B-9052 Zwijnaarde Belgium <mailto:guy.hermans@ablynx.com> guy.hermans@ablynx.com tel: fax: mobile: +32 (0)9 261 06 57 +32 (0)9 261 06 27 +32 (0)486 788 551 <https://www.plaxo.com/add_me?u=30065269879&v0=994426&k0=2009290972> Add me to your address book... <http://www.plaxo.com/signature> Want a signature like this?
