Re: What is a dump channel?

From: Randy T. Fischer <fischer1@mail.nih.gov>
Date: Fri Aug 12 2005 - 08:25:32 EST
Hi Guy,

At least for our lab, we use a ³dump² channel for exactly what it sounds
like- a ³trash dump².  We are interested primarily in human B cells, and in
some of our sick patients these can be quite rare in comparison to the other
types of peripheral blood cells.  So, to be able to more easily analyze
these rare populations, we label T-cells, NK-cells, monocytes, dendritic
cells and dead cells with a single fluorochrome/color and anything positive
in this channel is ³dumped² out of the populations to be analyzed.

Randy T. Fischer, NIH/NIAMS
B Cell Biology Group
9000 Rockville Pike
Bldg 10, Room 6D50
Bethesda, MD 20892
(301) 594-3537 (voice)
(301) 402-2209 (fax)



From: Guy Hermans <Guy.Hermans@ablynx.com>
Date: Thu, 11 Aug 2005 03:17:07 -0400
To: cyto-inbox
Subject: What is a dump channel?

Hi all,
 
After doing quite some reading on the list and in publications, I got the
impression various people use the term "dump channel" in reference to
different concepts/uses. Could some of the gurus out there clarify this one
for me and the rest of us out there?
 
thanks,
 
Guy
 
  
  
		  
	  
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Received on Fri Aug 12 15:18:00 2005

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