Re: Single Parameter Compensation

Elizabeth,

The simple answer is that there is no reason to do so.

Are you worried about other non-reagent fluorescence spilling into the one 
fluorochrome ala autofluorescence.  There are ways to deal with that.

Could you explain why you would want to apply compensation to a single 
fluorochrome?

Alan 
BD Biosciences
===========




"Kraus, Elizabeth" <elizabkr@BaylorHealth.edu>
07/07/05 01:49 PM
	To:	Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>
	cc:	(bcc: Alan Stall/SDCA/BDX)
	Subject:	Single Parameter Compensation



Flow Community:

Does anyone have a thought one way or another on applying compensation to 
a
sample with only one fluorochrome present?  Any thoughts or input would be
appreciated.

Thanks,

Elizabeth Kraus
Manager, Flow Cytometry Core Facility
B.I.I.R.
et.kraus@bhcs.com


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