Frances Gibson writes: <Am interested in looking at caspase activation by flow. Looked into this a few years ago when doing westerns for caspase activation, and didn't seem to be monoclonals fluorochrome confugated for flow that were available and used and data published. Does anyone have experience, information, references ? Any feedback would be great> We have extensive experience with the use of activated (cleaved) caspase-3 Ab. The polyclonal rabbit anti-caspase-3 Ab available from Cell Signaling works very well. We used it both, in conventional assays as well as in conjunction with ZENON Molecular Probes technology. E.g. see: Pozarowski et al., Interactions of fluorochrome-labeled caspase inhibitors with apoptotic cells. A caution in data interpretation. Cytometry 55A: 50-60, 2003. See also Huang et al at: http://www3.interscience.wiley.com/cgi-bin/fulltext/107632598/PDFSTART Zbigniew Darzynkiewicz, M.D., Ph.D. Brander Cancer Research Institute at NYMC 19 Bradhurst Avenue, Suite 2400 Hawthorne, N.Y. 10532 darzynk@nymc.edu http://www.darzynkiewicz.com/zbigniew/ -----Original Message----- From: Frances Gibson [mailto:fgibson@sgul.ac.uk] Sent: Wednesday, July 06, 2005 4:23 AM To: cyto-inbox Subject: anti-caspase antibodiesReceived on Thu Jul 7 14:58:00 2005
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