Dear all, I am trying to elutriate Jurkat cells. I keep getting clumps at the bottom of the elutriation chamber which results in poor fraction separation. I have been trying different approaches to minimize the cell agglomerate using Beckman chamber by: 1) passing cells through a syringe and a cell strainer to minimize cell adhesion 2) DNase treatment prior to cell loading 3) reducing the percentage of serum in the medium (standard RPMI) 4) adding EDTA to the medium 5) resuspend the cells in PBS instead of medium 6) Ficoll treatment prior to cell loading Does anybody have experience elutriating mammalian cells that could help me prevent the cell clumping? Thanks for any information you will provide me with Best regards Gabry Gabriella Rustici, PhD Laboratory of Receptor Biology and Gene Expression National Cancer Institute National Institutes of Health 301-435-2890 Phone 301-402-3134 Fax <mailto:rusticig@mail.nih.> <mailto:rusticig@mail.nih.gov> rusticig@mail.nih.gov Address for United States Postal Service Mail National Institutes of Health Advanced Technology Center, Room 133E 8717 Grovemont Circle Bethesda, MD 20892-4605 Address for UPS and FedEx National Institutes of Health Advanced Technology Center, Room 133E 8717 Grovemont Circle Gaithersburg, MD 20877Received on Tue Jun 28 13:58:00 2005
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