Just to clarify that Ken does not work for BD. He was at one time a consultant to BD, so he is not being unprofessional or trying to hide the truth. You best get your facts straight before calling someone unethical. David Matsuyama BD Biosciences |---------+---------------------------------------> | | owner-cytometry@flowcyt.cyto| | | .purdue.edu | | | | | | 06/16/2005 11:10 AM | | | | |---------+---------------------------------------> >---------------------------------------------------------------------------------------- ------------------------------------------------------| | | | To: undisclosed-recipients:; | | cc: (bcc: David Matsuyama/SANJ/BDX) | | Subject: | >---------------------------------------------------------------------------------------- ------------------------------------------------------| `From mailnull@flowcyt.cyto.purdue.edu Thu Jun 16 10:32:24 2005 Received: from flowcyt.cyto.purdue.edu (localhost.cyto.purdue.edu [127.0.0.1]) by flowcyt.cyto.purdue.edu (8.12.10/8.12.10) with ESMTP id j5GFWMvF072805 for <cytolist@flowcyt.cyto.purdue.edu>; Thu, 16 Jun 2005 10:32:22 -0500 (EST) (envelope-from mailnull@flowcyt.cyto.purdue.edu) Received: (from mailnull@localhost) by flowcyt.cyto.purdue.edu (8.12.10/8.12.10/Submit) id j5GFWMAZ072804 for cytolist; Thu, 16 Jun 2005 10:32:22 -0500 (EST) (envelope-from mailnull) Received: from splhost.spltrak.propagation.net (splhost.spltrak.propagation.net [66.221.123.92]) by flowcyt.cyto.purdue.edu (8.12.10/8.12.10) with ESMTP id j5GFWJI6072798 for <cytometry@flowcyt.cyto.purdue.edu>; Thu, 16 Jun 2005 10:32:20 -0500 (EST) (envelope-from overton@gcatinc.com) Received: from gcatinc.com (localhost [127.0.0.1]) by splhost.spltrak.propagation.net (8.11.6p2/8.11.6) with ESMTP id j5GFVJB02638; Thu, 16 Jun 2005 10:31:52 -0500 From: "W. Roy Overton, PhD" <overton@gcatinc.com> To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> Subject: Re: Coulter and Partec Date: Thu, 16 Jun 2005 10:31:19 +0700 Message-Id: <20050616103119.M19743@gcatinc.com> In-Reply-To: <035901c57115$64d85c00$f30456d1@organizak1y9ix> References: <42A9EFD6.6030209@pitt.edu> <035901c57115$64d85c00$f30456d1@organizak1y9ix> X-Mailer: WMail 1.64 20020516 X-OriginatingIP: 68.46.157.241 (overton) MIME-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 X-Spam-Score: -4.154 () BAYES_00,DATE_IN_PAST_12_24 X-Scanned-By: MIMEDefang 2.39 X-PMFLAGS: 34078848 0 1 72810.cnm Kenneth, It is unethical and unprofessional for a B-D employee, like yourself, to attempt to deceive the readers of this listserver by providing false and misleading competitive information and then to attempt to hide your true affiliation by calling yourself a "Flow Consultant" using your personal email and home address. Roy ************************************ W. Roy Overton, Ph.D. GCAT, Inc. 1629 Blue Spruce Drive Fort Collins, CO 80524 CO Office: 800-720-GCAT 970-224-2437 FAX: 970-472-9956 NJ Office: 856-478-0019 FAX: 856-478-9531 overton@gcatinc.com http://www.gcatinc.com ************************************ ---------- Original Message ----------- From: "Kenneth" <uffenheimer@mindspring.com> To: cyto-inbox Sent: Tue, 14 Jun 2005 12:15:07 -0700 Subject: Re: Coulter and Partec > Dear Paolo, > > I have studied both the Coulter and Partec systems and their > microplate sampler capabilities. Although the analyzers are > comparable, their microplate samplers do not rank well against the > BD High Throughput Sampler (HTS): > > Beckman Coulter FC 500 MPL > > a.. Must have 250 uL fluid in well (only one analysis possible, > since it requires175 uL to be aspirated per analysis) > b.. 96 well only, no 384 well > c.. Sample delivery via pressure control (low, med, high) - no > accurate volumetric control. > d.. SLOW - 35 seconds per well plus analysis time. Partec RobbyWell > a.. 96 well only, no 384 well > b.. Sample delivery via fixed volume loop, or partial loop > injection - pressure controlled drive into analyzer. > c.. SLOW - 2.4 hrs per 96 well plate. BD High Throughput Sampler > (HTS) > a.. User selectable 96 and 384 well microplate formats. > b.. Sample delivery rate and volume controlled via precision > syringe pump. > c.. User selectable acquire volume (2-200 uL) and rate (0.5 - 3.0 > uL/sec) > d.. FAST - less than 15 min per 96 well plate in "high throughput > mode". > e.. removes only 22 uL from the well, for up to 10 uL acquire > volume. > f.. User selectable mixing and wash parameters. The BD HTS is an > option for both the FACSCalibur (4 color) and LSR II (configurable > up to a dozen or more colors). I believe the HTS will be an option > for the FACSCanto (multi-color) in the near future. > > BD High Throughput Sampler Info: > http://www.bdbiosciences.com/immunocytometry_systems/support/training/online/ HTS_C/ > > http://www.bdbiosciences.com/immunocytometry_systems/products/display_product .php?keyID=92 > > Sincerely, > > Ken > > Kenneth F. Uffenheimer > Flow Consultant > 24185 Summit Woods Dr. > Los Gatos, CA 95033 > > email: uffenheimer@mindspring.com > phone: (408) 353-1401 > mobile: (408) 499-7150 > ----- Original Message ----- > From: paolo > To: Cytometry Mailing List > Sent: Friday, June 10, 2005 12:53 PM > Subject: Coulter and Partec > > Dear flow experts, Greetings to all of you. > > Our lab needs a 5 color (dual laser, 488 and 635) cytometer that > can read samples from a 96 well plate format, and I am looking for > some help in choosing between two instruments. > > To my knowledge, what is available in the US market at this time > is: (1) the Coulter Cytomics FC500 MPL and (2) the Partec CyFlow > Space equipped with the RobbyWell. > > Does anyone have any knowledge of these two? > > There are several elements that will influence the decision: > > 1) Quality: I was told that Partec uses microscope lenses to > collect/detect signal and this considerably increases the > sensitivity. Also they have absolute cells counting. > > 2) Price. the Partec cytometer is at least 20% less expensive > than Coulter's. Also for little more money it is possible to get one > additional detector to make it into a 6 color machine. > > 3) Service: Coulter is produced in the US and they have a large > customer base. Based on personal experience on the two XLs we have > now, Coulter's service is, just to be short, excellent. > Interestingly the cost of the service contract for the Partec > machines is less than Coulter's: the reason for this is that they > need less repairs (I was told). > > It will be quite an investment for our lab, so any suggestions, > comments etc will be greatly appreciated. > > Thanks > > Paolo > > ======================= > > aut viam inveniam aut faciam > > ======================= > > Paolo Piazza, PhD > > 425 Parran Hall > > Infectious Diseases and Microbiology > > GSPH - University of Pittsburgh > > tel (412) 624 - 3130 > > fax (412) 624 - 4953 > > e-mail paolo@pitt.edu ------- End of Original Message ------- ----------------------------------------- ******************************************************************* IMPORTANT MESSAGE FOR RECIPIENTS IN THE U.S.A.: This message may constitute an advertisement of a BD group's products or services or a solicitation of interest in them. If this is such a message and you would like to opt out of receiving future advertisements or solicitations from this BD group, please forward this e-mail to optoutbygroup@bd.com. ******************************************************************* This message (which includes any attachments) is intended only for the designated recipient(s). It may contain confidential or proprietary information and may be subject to the attorney-client privilege or other confidentiality protections. If you are not a designated recipient, you may not review, use, copy or distribute this message. If you receive this in error, please notify the sender by reply e-mail and delete this message. Thank you. ******************************************************************* Corporate Headquarters Mailing Address: BD (Becton, Dickinson and Company) 1 Becton Drive Franklin Lakes, NJ 07417 U.S.A. *******************************************************************Received on Fri Jun 17 15:18:00 2005
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