In addition to Ken's very informative study of the 96 well samplers I would like to point out that the BD HTS is not only very reliable (we have used them for over two years with negligible down time) it can be replaced in about 10 minutes on a FACS Calibur. I think it is relatively easy to remove from the LSR II as well. In case you have a problem you can not fix (a rare event with the HTS) just call BD's tech support and request a replacement unit while you send it back for repair. They generously offer this service but are VERY reluctant to fulfill it. Be persistent and discuss it with the instrument sales person before purchasing it. The HTS is shamelessly overpriced and they should meet high standards for $ 40,000. Akos ________________________________________________ Akos Szilvasi Flow Cytometry and Confocal Microscopy Core Facilities Novartis Institutes for Biomedical Research, Inc. (NIBRI) 100 Technology Square, Cambridge, MA 02139 akos.szilvasi@novartis.com tel 617 871 7177 fax 617 871 7051 "Kenneth" <uffenheimer@mindspring.com> 06/14/2005 03:15 PM To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> cc: (bcc: Akos Szilvasi/PH/Novartis) Subject: Re: Coulter and Partec Dear Paolo, I have studied both the Coulter and Partec systems and their microplate sampler capabilities. Although the analyzers are comparable, their microplate samplers do not rank well against the BD High Throughput Sampler (HTS): Beckman Coulter FC 500 MPL Must have 250 uL fluid in well (only one analysis possible, since it requires175 uL to be aspirated per analysis) 96 well only, no 384 well Sample delivery via pressure control (low, med, high) - no accurate volumetric control. SLOW - 35 seconds per well plus analysis time. Partec RobbyWell 96 well only, no 384 well Sample delivery via fixed volume loop, or partial loop injection - pressure controlled drive into analyzer. SLOW - 2.4 hrs per 96 well plate. BD High Throughput Sampler (HTS) User selectable 96 and 384 well microplate formats. Sample delivery rate and volume controlled via precision syringe pump. User selectable acquire volume (2-200 uL) and rate (0.5 - 3.0 uL/sec) FAST - less than 15 min per 96 well plate in "high throughput mode". removes only 22 uL from the well, for up to 10 uL acquire volume. User selectable mixing and wash parameters. The BD HTS is an option for both the FACSCalibur (4 color) and LSR II (configurable up to a dozen or more colors). I believe the HTS will be an option for the FACSCanto (multi-color) in the near future. BD High Throughput Sampler Info: http://www.bdbiosciences.com/immunocytometry_systems/support/training/online/HTS_C/ http://www.bdbiosciences.com/immunocytometry_systems/products/display_product.php?keyID=92 Sincerely, Ken Kenneth F. Uffenheimer Flow Consultant 24185 Summit Woods Dr. Los Gatos, CA 95033 email: uffenheimer@mindspring.com phone: (408) 353-1401 mobile: (408) 499-7150 ----- Original Message ----- From: paolo To: cyto-inbox Sent: Friday, June 10, 2005 12:53 PM Subject: Coulter and Partec Dear flow experts, Greetings to all of you. Our lab needs a 5 color (dual laser, 488 and 635) cytometer that can read samples from a 96 well plate format, and I am looking for some help in choosing between two instruments. To my knowledge, what is available in the US market at this time is: (1) the Coulter Cytomics FC500 MPL and (2) the Partec CyFlow Space equipped with the RobbyWell. Does anyone have any knowledge of these two? There are several elements that will influence the decision: 1) Quality: I was told that Partec uses microscope lenses to collect/detect signal and this considerably increases the sensitivity. Also they have absolute cells counting. 2) Price. the Partec cytometer is at least 20% less expensive than Coulter's. Also for little more money it is possible to get one additional detector to make it into a 6 color machine. 3) Service: Coulter is produced in the US and they have a large customer base. Based on personal experience on the two XLs we have now, Coulter's service is, just to be short, excellent. Interestingly the cost of the service contract for the Partec machines is less than Coulter's: the reason for this is that they need less repairs (I was told). It will be quite an investment for our lab, so any suggestions, comments etc will be greatly appreciated. Thanks Paolo ======================= aut viam inveniam aut faciam ======================= Paolo Piazza, PhD 425 Parran Hall Infectious Diseases and Microbiology GSPH - University of Pittsburgh tel (412) 624 ? 3130 fax (412) 624 ? 4953 e-mail paolo@pitt.eduReceived on Thu Jun 16 16:18:00 2005
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