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From: "W. Roy Overton, PhD" <overton@gcatinc.com>
To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>
Subject: Re: Coulter and Partec
Date: Thu, 16 Jun 2005 10:31:19 +0700
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Kenneth,
It is unethical and unprofessional for a B-D employee, like yourself, to 
attempt to deceive the readers of this listserver by providing false and 
misleading competitive information and then to attempt to hide your true 
affiliation by calling yourself a "Flow Consultant" using your personal 
email and home address.

Roy

************************************
W. Roy Overton, Ph.D.
GCAT, Inc.
1629 Blue Spruce Drive
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CO Office: 800-720-GCAT
	   970-224-2437
      FAX: 970-472-9956
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     overton@gcatinc.com

      http://www.gcatinc.com
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---------- Original Message -----------
From: "Kenneth" <uffenheimer@mindspring.com>
To: cyto-inbox
Sent: Tue, 14 Jun 2005 12:15:07 -0700
Subject: Re: Coulter and Partec

> Dear Paolo,
> 
> I have studied both the Coulter and Partec systems and their 
> microplate sampler capabilities.  Although the analyzers are 
> comparable, their microplate samplers do not rank well against the 
> BD High Throughput Sampler (HTS):
> 
> Beckman Coulter FC 500 MPL
> 
>   a.. Must have 250 uL fluid in well (only one analysis possible,
>  since it requires175 uL to be aspirated per analysis) 
>   b.. 96 well only, no 384 well 
>   c.. Sample delivery via pressure control (low, med, high) - no 
> accurate volumetric control. 
>   d.. SLOW - 35 seconds per well plus analysis time. Partec RobbyWell
>   a.. 96 well only, no 384 well 
>   b.. Sample delivery via fixed volume loop, or partial loop 
> injection - pressure controlled drive into analyzer. 
>   c.. SLOW - 2.4 hrs per 96 well plate. BD High Throughput Sampler 
> (HTS)
>   a.. User selectable 96 and 384 well microplate formats. 
>   b.. Sample delivery rate and volume controlled via precision 
> syringe pump. 
>   c.. User selectable acquire volume (2-200 uL) and rate (0.5 - 3.0 
> uL/sec) 
>   d.. FAST - less than 15 min per 96 well plate in "high throughput 
> mode". 
>   e.. removes only 22 uL from the well, for up to 10 uL acquire 
> volume. 
>   f.. User selectable mixing and wash parameters. The BD HTS is an 
> option for both the FACSCalibur (4 color) and LSR II (configurable 
> up to a dozen or more colors).  I believe the HTS will be an option 
> for the FACSCanto (multi-color) in the near future.
> 
> BD High Throughput Sampler Info:
> 
http://www.bdbiosciences.com/immunocytometry_systems/support/training/online/
HTS_C/
> 
> 
http://www.bdbiosciences.com/immunocytometry_systems/products/display_product
.php?keyID=92
> 
> Sincerely,
> 
> Ken
> 
> Kenneth F. Uffenheimer
> Flow Consultant
> 24185 Summit Woods Dr.
> Los Gatos, CA 95033
> 
> email: uffenheimer@mindspring.com
> phone: (408) 353-1401
> mobile: (408) 499-7150
>   ----- Original Message ----- 
>   From: paolo 
>   To: Cytometry Mailing List 
>   Sent: Friday, June 10, 2005 12:53 PM
>   Subject: Coulter and Partec
> 
>   Dear flow experts, Greetings to all  of you.
> 
>   Our lab needs a 5 color (dual laser, 488 and 635)  cytometer that 
> can read samples from a 96 well plate format, and I am looking for 
> some help in choosing between two instruments. 
> 
>   To my knowledge, what is available in the US market at this time 
> is: (1) the Coulter Cytomics FC500 MPL and (2) the Partec CyFlow 
> Space equipped with the RobbyWell. 
> 
>   Does anyone have any knowledge of these two? 
> 
>   There are several elements that will influence the decision:
> 
>   1) Quality: I was told that Partec uses microscope lenses to 
> collect/detect signal and this considerably  increases the 
> sensitivity. Also they have absolute cells counting. 
> 
>   2) Price.  the Partec cytometer is at least  20% less expensive 
> than	Coulter's. Also for little more money it is possible to get one 
> additional detector to make it into a 6 color machine.  
> 
>   3) Service: Coulter is produced in the US and they have a large 
> customer base. Based on personal experience on the two XLs we have 
> now, Coulter's service is,  just to be short, excellent. 
> Interestingly the cost of the service contract for the Partec 
> machines is less than Coulter's: the reason for this is that they 
> need less repairs (I was told).
> 
>   It will be quite an investment for our lab, so any suggestions,
>  comments etc  will be greatly appreciated.
> 
>   Thanks
> 
>   Paolo
> 
>   =======================
> 
>   aut viam inveniam aut faciam
> 
>   =======================
> 
>   Paolo Piazza, PhD
> 
>   425 Parran Hall
> 
>   Infectious Diseases and Microbiology
> 
>   GSPH - University of Pittsburgh
> 
>   tel (412) 624 - 3130
> 
>   fax (412) 624 - 4953
> 
>   e-mail   paolo@pitt.edu
------- End of Original Message -------