RE: Use of 7-AAD on a FACS Array, with fixation??

Hi Patricia,

 

The Array resuspended cells prior to sample uptake by "pipetting" up and
down (see user prefs), then "pipette up" a given volume and injects this
into the sample pathway, which is a closed cuvette-straight-to-waste type of
deal. I guess there are no aerosols involved in the same sense as in
sorters/jet-in-air devices, maybe a tiny bit if you're trying to "pipette
up" volumes larger than the wells actually contains.

 

I mostly use PI or TOPRO3 on the Array, have done 7-AAD sometimes - never
tried combining any of those with fixation, as my understanding is none of
these can be used on fixed cells: everything will probably light up.

 

Guy

 







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-----Original Message-----
From: Patricia Echeagaray [mailto:pechado@hotmail.com] 
Sent: Wednesday, May 25, 2005 6:22 PM
To: cyto-inbox
Subject: Use of 7-AAD on a FACS Array, with fixation??

 


All,

Doing a new procedure and a question came up.....

Is it possible to use 7-AAD on a Facs Array, using infected cells WITH
fixation? 
OR must they be analyzed without the benefit of being fixed? 
Does the FacsArray aerosolize (sp?) the sample?
Does 7-AAD tolerate any fixation ? 

TIA!!

Patti in damp. rainy MD 
(but the flowers (as in plants) are happy, if the flowers (as in
cytometrists) are not!)