The Mystery of The High Throughput Sampler, The Sampler Port, and the Clumped Cells

Good Afternoon Everyone
 
I want to find out why the sample port clogs when I use the HTS but it works fine when doing Manual Tube Loading (MTL) 
I have an assay that runs perfectly when I use tubes to load the samples.  However, the Calibur sample port clogs constantly when I use the HTS. 
Looking at the samples under the microscope, we found that there are some clumped cells. 
We think that the boost pressure of the HTS is higher than the boost pressure of the Calibur when doing MTL
 
Any comments are welcome
 
Thanks

Rafael Villasmil 
Associate Scientist I

Medimmune Inc 
Phone Number (301) 398-4478
One MedImmune Way
Gaithersburg, MD 20878 

"Accept the challenges, so that you may feel the exhilaration of victory."

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