Dear Flowers, Can I use BD's Trucount tubes for Ficoll separated human PBMCs [used BD's CPT tubes(Cell Preparation Tube)for PBMC preparation] to get absolutes of different cell types in humans. My FACS panel includes 4-5 colors of different human cell surface markers using CPT separated human PBMCs. Could any of you suggest any established bead spike method (Vendor and bead size) for absolute counts. Thanks in advance for your valuable time and suggestions. I would like to thank each and everybody for suggesting various options and dyes for my previous question about dead cell discriminator using violet and red lasers and also the Purdue University Group responsible for this list. Saraswathi Naravula Scientist II, Dept of Discovery Research 901 California Avenue Palo Alto, CA 94304-1104 Ph: 650-496-6550 Fax: 650-496-1200 saraswathi.naravula@dnax.org ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete.Received on Mon May 2 16:01:56 2005
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