Hello everyone! I would like to know if anybody out there that has done bacterial cell sorting can be kind enough to share the sort settings used on their machines. We have a Vantage SE DIVA and we are trying to sort E.coli based on porphyrin expression. In terms of the optics configuration, it looked OK, like we can distintinguish the bacteria from the noise, no problem, but as soon as I turn the ddf on I can't get rid of the noise. (By the way the neurtal density filter was removed as suggested by Mark) (The first try was done with the 70 um nozzzle, at 49200Hz, 31 psi. , (Hey, I know I should have tried a smaller one, but I guess we wanted to test the limits ...:)) , and I wonder if using a smaller size nozzle or using other settings can help on this? So, If anybody can provide suggestions as to which size nozzle, frequencies and buffers to use we will greatly appreciate it. Thanks in advance Johana Johana Melendez,MS Research Associate Flow Cytometry Laboratory H. Lee Moffitt Cancer Center 12902 Magnolia Dr. Tampa, Florida 33612-9497 Phone (813) 745-6610 Lab. phone (813) 972-8400 x. 2005 Fax (813) 903-7140 <<Melendez, Johana.vcf>> ----------------------------------------- ########################################################################### ## This transmission may be confidential or protected from disclosure and is only for review and use by the intended recipient. Access by anyone else is unauthorized. Any unauthorized reader is hereby notified that any review, use, dissemination, disclosure or copying of this information, or any act or omission taken in reliance on it, is prohibited and may be unlawful. If you received this transmission in error, please notify the sender immediately. Thank you. ######################################### ####################################
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