Judith: Your email does not make it clear how (by what parameters) you are trying to identify the beads. Most of these beads have one or more parameters, often in fluorescence, in which they are clearly distinguishable from the "RBC/WBC clump" and can be gated upon for deriving an absolute count. That said, we have been using a product from Caltag which identifies the beads based on unique light scatter characteristics. These beads are nice because they actually consist of a mixture of two beads, of slightly different size, the ratio of which provides an internal control for bead mixing and pipeting problems. For further information see Cytometry Part B 57B:47-52 (2004). The beads described in the reference are described as being from a manufacturer in Spain, but this is the same product offered by Caltag. Wayne F. Green, Ph. D. Flow Cytometry Core Laboratory University of Utah HSC Telephone 801 585-7382 Fax 801 581-7183 -----Original Message----- From: Stewart, Judith [mailto:Judith_Stewart@URMC.Rochester.edu] Sent: Wednesday, March 30, 2005 8:17 AM To: cyto-inbox Subject: absolute count beads I'm looking for a product that will allow me to get an absolute count when running a whole blood prep for platelet analysis. I've tried spherotech, BD and Coulter but they all end up in the RBC/WBC clump and I can't isolate them. I hoping somebody out there knows a source for a product whose size is <5um and comes with an absolute count.Received on Thu Mar 31 13:38:00 2005
This archive was generated by hypermail 2.1.8 : Sat Jan 14 2006 - 22:03:41 EST
