RE: looking for the right flow cytometer

The Guava PCA may meet his needs.  It is designed for analysis of animal
cells, but Guava would probably bring an instrument for a demo so he could
see if it would work for his cells.  The instrument does counts without an
internal standard, comes with two fluorescence detectors, comes with either
a 488 or 532 nm laser, and has a 96 well plate reader option.
www.guavatechnologies.com <http://www.guavatechnologies.com> 
Nick Abu-Absi 
Process Development Scientist 
Chiron Corporation 
4560 Horton St. 
M/S M-200 
Emeryville, CA 94608 
Lab: (510)923-7421 
Fax: (510)923-4116 

-----Original Message-----
From: Oughton, Julie [mailto:julie.oughton@oregonstate.edu]
Sent: Thursday, March 17, 2005 8:17 AM
To: cyto-inbox
Subject: looking for the right flow cytometer



Hello!


I manage a flow cytometry core lab at Oregon State University. I have an
investigator who has an urgent need for a highly specialized instrument to
enumerate bacteria. He is interested in only one readout-cell
concentration-the number of bacteria per mL-without the need for an internal
standard. He is most interested in purchasing an instrument due to the high
throughput of samples that are processed on a daily basis. Currently, his
lab is using microscopic examination to enumerate the number of bacteria in
his cultures.


In his words, the ideal instrument would be equipped with a single detector
[in order to use a DNA stain to identify his bacteria] and capable of
detecting bacteria down to 0.4 micron in size. So, in his mind, an
instrument that measures a standard volume would be ideal. If the instrument
could sample from microtiter plates, he would be ecstatic. Of course, ease
of use would also be desirable since there will be a number of lab personnel
using this instrument.


So, if possible, I would like to the pick the brains of this group. I'm sure
there must be someone out there who can recommend an instrument that meets
our needs. We would entertain all recommendations and comments. Sample
throughput has been so high in his lab that he has placed a high priority on
purchasing such an instrument.


Thanks in advance

Julie Oughton

Oregon State University