Dear friends, I want to begin titrating TMRM for mitochondrial membrane potential estimation for apoptosis assays, and found that the supplied data sheet from sigma is very scarce, and mol probes does not give much info either. I did find in the net a datasheet that warned that TMRM is very unstable in solution and should be used immediately. Measuring such small, micro-g amounts for individual experiments is impossible for the normal lab (or not???), so how do you use TMRM then? How long does it keep stable "enough" in solution? I would be very grateful if anyone with experience with TMRM could advice me a little on the matter. Thanks in advance, Uriel. PS:I intend to use DMSO as solvent.Received on Thu Mar 24 13:38:00 2005
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