Flow jocks, biochem freaks & geeks, I've endeavoured to conjugate some proteins myself for use in the lower channels of my FACSarray: excitation using a solid-state laser @532nm, detection using a 585/42 filter. I can use PE, of course, but would rather have a smaller dye molecule and/or cheaper alternative handy as well. Anyone have useful hints as to brightest/easiest to conjugate/most photostable dye to use for that combo? I've looked into the Alexa dyes, but most of them don't have a very high stokes shifts so I have to optimize either excitation and pick up only the emission tail, or deal with suboptimal excitation. In that spectral region, none of them seems to match my requirements very well (based on normalised spectra only, mind you). I've also looked hard into TAMRA and TRITC, both of which seem to match my spectral requirements perfectly. Probes has several isoforms and mixes Which one to chose? Any reason why these don't seem to be used very frequently for FACS? I googled up an NIH webpage http://home.ncifcrf.gov/ccr/flowcore/projects.htm (Mario?) which links to a comparison of such dyes, but the page with the comparison http://home.ncifcrf.gov/ccr/flowcore/DPSS532.htm is no longer there. Thanks, Guy <http://www.ablynx.com> Next generation therapeutic antibodies Guy Hermans, PhD Project Leader Ablynx NV Technologiepark 4 B-9052 Zwijnaarde Belgium <mailto:guy.hermans@ablynx.com> guy.hermans@ablynx.com tel: fax: mobile: +32 (0)9 261 06 23 +32 (0)9 261 06 27 +32 (0)486 788 551 <https://www.plaxo.com/add_me?u=30065269879&v0=994426&k0=2009290972> Add me to your address book... <http://www.plaxo.com/signature> Want a signature like this?
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