Finding the 458nm laser line of a Coherent innova Spectrum Laser70 on FACSVantageSE

Hi FlowCytonauts,

I ran into trouble finding the 458nm laser line of our Innova Spectrum70 
Laser on our FACSVantage SE for ECFP analysis.
Actually I can tune to 6 lines below the very strong 488nm line. (UV is 
probably not visible)

The Innova handbook says:
70C-Spectrum:
488.0: 0.25Watt;
476.5: 0.10
472.2: ----
465.8: ----
457.9: 0.03
Multiline UV: 0.05
So which one is the 458 ?. The first visible one ?

Unfortunately I currently have no strong ECFP expressing cell line for 
fishing in the dark.
Are there any beads that are somewhat specific for that laserline ?
I tried Sphero-Yellow bright with not much success...
I am running the laser as  secondary laser (488 primary, outsplitted UV 
as laser3).
I used a 649LP filter and collected the reflected light with a special 
473/19 filter (as discussed previously in that group).

Any help is greatly appreciated ...

/Alex






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