Sara, If your looking for standard Immunophenotyping I would suggest you use the 4-color cocktails & protocols that are available now (BD Multitest) and add 1 or 2 colors at choice, you’ll need to perform some (but less than for all 6 colors) titration experiments. If you dispose of the DIVA soft & a Canto or LSR II, auto compensation should do the trick and you will be able to start soon. Although indeed if for clinical purposes, you will have some hard validation work coming your way. Anyway Good Luck. Frédéric Clement Ghent University _____ Van: Markestad, Sara [mailto:SMarkestad@sjha.org] Verzonden: maandag 21 februari 2005 19:58 Aan: Cytometry Mailing List Onderwerp: 5-6 color immunophenotyping Hello Flowers! Does any one know of any good references, protocols, procedures that are available on this topic? We are looking to start 5-6 color immunophenotyping here in the next couple of months and I would like to see what others have done or try before I attempt this myself. Thanks for your help! Sara Markestad, MT (ASCP) St. Joseph's Hospital of Atlanta 404-851-7744 _____ Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph’s Health System, Inc.Received on Thu Feb 24 15:38:00 2005
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