Re: Problem of software compensation with Flowjo 6.0

From: Adam Treister <adam@treestar.com>
Date: Sat Feb 19 2005 - 00:49:34 EST
Alex,

The feature changed slightly in the 6.0 release.  Now, the compensation 
matrix is by default saved within the workspace, instead of in a 
separate file.	If you need to export the file (as is the only option 
in the older versions), there is an additional menu item for Edit / 
Save Matrix, under Platform >> Compensate Sample.

Adam


On Feb 18, 2005, at 6:08 AM, Li Chen wrote:

> Hi Flowers,
>
>	 Sometimes it is not easy to do the hardware compensation when
> dealing with very small number of cells. I collect my FACS data with
> BDCalibur  and try to do software compensation using FlowJo 6.0 on Mac
> (OS 9.0). I followed the manual step by step: define the matrix using
> single color sample--> save the matrix --> load the matrix--> apply it
> to my samples of interest. What troubles me is that after I saved the
> matrix I couldn't load it.  I couldn't find its location using the
> search function in the computer although it appeared on the manual 
> under
> COMPENSATION BAR. I wonder if it is a bug for this software (I have no
> problem with Window system using the demo data). Does anyone out there
> have some ideas?
>
> Alex	

---------------------------------------------
Adam Treister
Tree Star, Inc.
340 A St.
Ashland OR 97520
1-800-366-6045
---------------------------------------------
Received on Mon Feb 21 14:18:00 2005

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