Quick question: We are using a FACSAria with a 407nm laser. We are wanting to get a dye that is excited at 407nm and is as bright as possible (when conjugated to an antibody with MP's Zenon reagents). The current PMT filters are at 530/30 nm and 450/40 nm. We could change those if that would help. We have tried Alexa 430 and found it to be rather dull. Any thoughts about whether Alexa 405 or Cascade Blue would be any brighter? Thanks. Alice Alice L. Givan Englert Cell Analysis Laboratory of the Norris Cotton Cancer Center Dartmouth Medical School Lebanon, NH 03756 USA tel 603-650-7661 fax 603-650-6130 givan@dartmouth.edu www.dartmouth.edu/~celllabReceived on Mon Jan 31 14:58:00 2005
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