Dear friends, Could it be possible to be serious with the CMXRos probe, at least to what concern the detection of the mitochondrial membrane potential after cell fixation. Please look at : The use of chloromethyl-X-rosamine (Mitotracker Red) to measure loss of mitochondrial membrane potential in apoptotic cells is incompatible with cell fixation Cytometry Volume 36, Issue 4, Date: 1 August 1999, Pages: 355-358 and if you wanted to carry experiments, please try controls with either uncoupling agent (CCCP, FCCP, nig + val) or in regulating volontary (calibration) the size of the mitochondrial potential. You will normally get a fast answer to your question and ridiculous, unexpected results... Sincerely yours Patrice Dr. Petit Patrice X. INSTITUT COCHIN INSERM U.567, CNRS UMR 8104, IFR 116 Department of Genetic, Development and Molecular Pathology Team 5 "Cancer, Apoptosis and Mitochondria" CHU Cochin Port-Royal 24, rue du Faubourg Saint-Jacques F-75014 Paris, France. Tel: 33 01 44 41 24 11 Fax: 33 01 44 41 24 21 E-mail: pxpetit@cochin.inserm.fr http://www.cochin.inserm.fr € This e-mail is confidential and should not be used by anyone who is not the original intended recipient. If you have received this e-mail in error please inform the sender and delete it from your mailbox or any other storage mechanism €Received on Wed Jan 12 14:38:00 2005
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