Purdue Cytometry Mailing List By Subject
- Howard Shapiro (Wed Sep 29 2004 - 22:40:33 EST)
- "A stranger is a Friend I have not meet yet"./Spectral Overlap
- "Analysis of Microbial Cells at the Single Cell Level"
- "Analysis of Microbial Cells at the Single Cell Level" - Correct Address
- "Cellular Astronomy" - The Next Small Thing?
- % of Lymphocyte by hematology counter vs. FLOW
- % of Lymphocyte by hematology counter vs. FLOW: FOLLOW UP
- (almost) sterile sorting
- (anti) Commercial Posting:)
- (Fwd) CFP and Announcement - International Journal of Medical
- (Fwd) Positions to post
- (no subject)
- 10th Leipziger Workshop, 2nd announcement
- 1301 telomere length
- 14-bit ADC to 18-bit scale... why bother?
- 17th Annual Clinical Flow Cytometry Course in Pittsburgh, Sept 5- 10
- 2004 - A quality year in Cytometry: Review
- 200u sorting on VantageSE
- 200u sorting summary
- 3D Graphics for flow data display
- 405 EXCITABLE DYES
- 405 nm laser // time lapse microscopy
- 405 nm vs. 532 nm laser
- 488/UV multiline laser
- 4th EWGCCA EuroConf, Sep.23-25, Mol, Belgium
- 5 color flow
- 5 or 6 colour reagents/panels on CYAN
- 532nm Excitation
- 6-NBDG, advice needed
- 600th person does survey
- 7AAD
- 7AAD : other viability options
- 7AAD and pyronin Y
- 7AAD or PI with APC, PC7, APC-Cy7 conjugates
- 96-well plate autosampler
- 96well PCR plates on the Aria
- =??Q?=BB=D8=B8=B4: Activation of platelets?=
- ?BLASTIC NK LYMPHOMA/LEUKAEMIA
- [ CSF1 and PDGF receptor levels? ]
- [ Fast sorter recommendation? ]
- [bacterial stably expressing GFP/YFP?]
- [BRdU in LOVO cells]
- [CD57 on T Cells]
- [Cell Cycle phase in subpopulations]
- [Compensation with different sized populations]
- [Cytometer Purchase advice?]
- [DNA dye exclusion viability test question]
- [DNA Staining of cells with intracellular bacteria]
- [Functional Analysis ISAC Workshop]
- [Fwd: N2 assay]
- [Hoechst / CD34 protocol ? ]
- [ISAC Workshop - apoptotic signal transduction]
- [MCSF-1 and PDGF receptors on smooth muscle cells]
- [Memory B cell in CVID]
- [MFI vs. percent positive ? ]
- [Neutrophil functional markers?]
- [Platelet analysis in from whole blood samples]
- [Reagent source?]
- [Sapphire laser opinions]
- [Surface marker quantitation questions]
- [TRUCOUNT for mouse WBC?]
- ```NK cells viability post sorting on FACSAria
- A Little Help From my Friends/ Spectrlal overlap
- A manufacturer's view of the instrumental calibration controversy
- a new home for an old Elite
- A Tutorial on WinMDI
- Ab PHAGE LIBRARY
- about the marker for RAG -/- mice
- about the time point in the BrdU cell cycle experiment
- absolute vs ratio, small comment
- Abstract Deadline for the Clinical Cytometry Meeting AUG 15th
- Academic Institution User Fee Survey Revisited
- ACAS Ultima confocal laser cytometer/SerCon
- ACAS Ultima confocal laser cytometer/SerCon-service rep
- Accutase for detachment for cell monolayers
- ACDU Error Rate
- Acridine Orange
- Activated neutrophils/eosinophils?
- Activation of platelets
- Adult peripheral blood
- Advanced Courses in Flow Cytometry in NY City in September
- Advice needed
- Advice on how to prepare adipocytes from rats to perform flow cytometric analyses
- AGGRESSIVE ?LYMPHOMA CASE
- Alexa Fluor 680/700 HeNe excitation
- Alice Givan and Howard Shapiro Seminar at UMass
- alignment of Coulter Elite
- Alkaline Phosphatase staining
- Aloha...and thanks!
- Alpha Beta TCR staining
- alternate source for Ortho Permeafix?
- alternative T cell activation markers
- ALTERNATIVE T-CELL ACTIVATION MARKERS
- an update
- Analisis of cell proliferation with CFSE
- analysing ROS?
- analysis of mixed primary cultures of neurons
- Analysis Software
- analysis vs sorting and nozzle sizes: FACSAria
- analyzing nuclear proteins by flow cytometry
- Andy Beavis - are you out there?
- Annexin binding on platelets
- Annexin on PBMC - real or false positives?
- Annexin on PBMC - real or false positives? - Summary of responses
- Annexin staining with platelets
- Annexin V
- Annexin V and caspase 3 protocol
- annexin V and monos
- annexin V on monocytes
- annexin V staining of peripherAL BLOOD MONONUCLEAR CELLS
- Annexin V vs. VAD-FMK
- Announce: Multiplex Assay Meeting UK
- Announcement, 10th Leipziger Workshop
- Annual Research Course in Flow Cytometry
- Another Acridine Orange Question
- Another CDC ORISE position
- anti Pichia antibodies
- anti-Flk-1 antibody options
- anti-human Notch1 antibody for flow
- anti-human-alpha4beta7
- anti-IL-6 antibody against not-recombinant IL-6
- anti-mouse CD18 and CD45
- anti-mouse FasL antibody
- anti-rat CD19 cross reacting mAb
- Anti-sheep MoAbs
- anti-TGF-bata antibody
- anti-TNF antibody
- antibacterial peptides
- Antibody against EPO
- antibody conjugated APC-Cy7
- Antibody dilutions
- antibody for flow-FISH
- Antibody Request
- Antibody titrations (2004)
- Antibody titrations (2004...2005...2006... and beyond)
- Antibody titrations-1 question
- Antibody-based uptake assay
- Antwort: Gating for apoptosis
- Antwort: Green fluorescent stain for K562 cells
- Antwort: maximizing recovery of paraformaldehyde-fixed cells
- Any experience of TRUE undifferentiated acute leukemia?
- Any one working with mouse blood?
- Any one working with mouse blood? -correction
- Any suggestion for selection of ICAM-1 (CD54) antibody?
- anybody using the Chroma Technology filters?
- Anyone used DiD
- Anyone using Cellquest on a Mac.
- anything better than Alexa 430?
- APC
- APC-Cy7 conjugated Streptavidin
- APODIRECT flow cytometry kit (Phoenix Flow Systems)
- apoptosis
- Apoptosis analysis
- Apoptosis and trypsin
- apoptosis in fresh PBMCs
- Apoptosis in peripheral blood lymphocytes (Tunel assay)
- Apoptosis Workshop-ISAC
- Apoptosis, Tunel assay in CD4 and CD8 T cells
- Applescript and CellQuest Pro
- Aramark CAPP
- Aria ideal max. sort rate w/ viable cells
- Aria medium and low pressure sorting
- Aria nozzle issues revisited
- Aria nozzle summary - Thanks
- Aria nozzles
- Aria sorting into 96 well plates
- Aria Stability
- Aria Users please read:
- Aria-ACDU adaptor for PCR rack?
- aseptic sort set up on Aria
- ask the Profis
- aspecific PE-staining on neutrophils
- AT1 expressing cell line.
- ATG and OKT3 determination / depleting
- Autoflourescence as "control" - finally an answer?
- Autofluorescence blocking
- Autofluorescence quenching
- Autofluorescence quenching - Clarification
- Autosampler for Facscalibur with temperature control - alternatives to Cytek?
- AW: Beads for cell size measurements
- Azide and calcium flux
- B-cell Markers on EBV Transformed B-Cell lines
- Bacillus spore permeabilisation and viability assessment
- Back doors to Overlays
- bacteria & low pH / high salt
- bacterial permeabilisation
- Basic and Advanced Flow Cytometry Courses in Denver
- Basic problem with RBC lysis
- Basic rhesus macaque cytometry
- Bayer Flow Cytometer
- BD CompBeads
- BD FACS Caliber
- Beads
- beads / cells/ml
- Beads for cell size measurements
- Beckman Coulter XL
- Best Secondary Reagents for Rabbit Polyclonal Abs
- Bio-containment
- BioE Vitalyse
- Biohazard containment
- biohazard sorting on Aria
- biohazard sorting on Aria - follow up
- Biohazardous sorting on an Aria
- BioRad Bryte HS
- Biorad confocal users
- BiOS 2005 Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues II (BO124).
- BiOS Meeting Announcement
- Biosafety and sorting infectious samples
- Biosafety of sorting revisited
- blood CD154 staining
- Blue laser Guava
- BM Biopsy for Flow
- bone marrow core biopsy processing
- Boston Users Group for Cytometry / CFAR HIV Meeting Announcement
- Boston Users Group for Cytometry HIV Meeting Announcement
- Boston Users Group for Cytometry Web Site
- Boston-Flow Cytometry Job Opening
- Bragging rights....!!
- brains
- Bravo, Bravo ISAC XXII
- BrdU
- BrdU and activated T cells in vivo
- BrdU and H33342
- brdu and saponin
- Brdu PI
- BrdU staining and surface antigen
- Breast milk lymphocytes
- Bug/feature in FC500 File naming - loss of data
- c-myc anti-mouse mAB
- Ca flux in frozen PBMC
- Ca for loading cells
- Ca-Flux
- caco cells and bacterial attachment
- Calcium ratio imaging events
- calibration beads analisis
- Calibration emission lamp for spectral confocal QA
- Calibur Crash Summary
- Calibur Crashes
- Can cells for flow be frozen?
- Can I do intracellular staining with biotin antibody ?
- Can some one offer an answer to my problem
- CAP checklist question FLO.30720
- Cardiomyocyte marker?
- Cardiomyocytes
- Carry over of fluorescent probes and stains to subsequent samples
- Cascade Cytometry User's Group meeting on Jan. 4
- Caspase activity in lymphocytes
- caspase activity in lymphs
- CBA quesion
- CBA question
- CBA versus flowcytomix
- CBA versus flowmix
- CCR3 and CCR10
- CCR5 mAb that works on fixed cells
- CCS meeting coming up
- CD 8
- CD antigen question
- CD133 Antibodies
- CD133 in paraffin/frozen tissue sections
- CD15 staining...
- CD16 in cynomolgus monkeys
- CD1a+ cells in LBA
- CD27 Staining
- CD3-CD4+
- CD34
- CD4 and CD8 coexpression problem
- CD4+ T cell enumeration using non-traditional cytometers
- CD4+//CD8+ in apparently normal individual
- CD4+CD25bright
- CD4-CD8- T cells in pediatric AIDS
- CD4/CD45RB sorts - High speed sorting
- CD45 expression in mouse lung
- CD45 versus SSC in mice bone marrow: summary
- CD64
- CD69 timing
- CD8 availability?
- CD8 available upon request
- CD94 and dog NK cells
- CDw32+ L cells
- CEDA,SE to replace CFDA for cell division
- cell activation markers
- cell alkaline dissociation for the chromosome aberration analysis
- cell clumping
- Cell Counter
- cell cycle analysis
- Cell Cycle Analysis by Centrifugal Elutriation
- cell cycle analysis in 96 well format
- cell cycle analysis of Jurkat
- Cell cycle analysis with DRAQ5
- Cell Cycle on Aria
- cell cycle on Aria answers
- Cell fixation after Annexin V labelling ?
- cell line on FACScalibur
- Cell size and FSC
- cell sorter and stem cell
- Cell sorter for sale (UK)
- Cell sorting with viability !! will it be possible?
- Cell Tracker Orange Alternative
- Cellquest Pro files into Cellquest
- CellQuest Pro Question
- CellQuest problem
- CellTrace Far Red for in vivo cell tracking
- Cellular Astronomy
- CFDA SE compensation control
- CFP and YFP beads
- CFP on LSR II/Qdots
- CFSE
- CFSE part 2
- cfse quenching?
- CFSE staining for spleen/lymph node cells
- cheapish books
- cheapish books AND Not so cheapish ones.....
- Check out the latest ISAC blog!!!
- Chicago User's Group (ChUG)
- Chris Threeton
- Clinical Flow Course Pittsburgh Sept 5-10
- Clinical Flow Cytometry Course
- Clinical Flow Cytometry Instrument - *Please Respond*
- Clinical Flow Cytometry Instrument - Please Respond!
- Clinical Flow Cytometry Instruments
- Clinical Flow Cytometry Position
- Clinical Flow Techologist Position
- CML and sorting
- CML panel
- COLD AGGLUTININS
- Column dye
- Comments I have recieved about ZAP-70
- Commercial polyclonal antibody prep
- Compbeads and Zenon kits
- Compensation Question
- Compensation Question Summary
- compensation videos
- COMPETITION COMMISSION CLEARS ZEISS/BIO-RAD MERGER
- Complimentary subscription of CELL CYCLE to some members of the scientific community
- CompuCyte
- Computer needed
- conferences
- Confocal Instability-shocking observation
- Confocal microscope Instability
- Confocal microscope Instability #2
- Confocal QA system stability --Summary responses
- Confocal Shape changes in Round beads
- Confocal spectral calibration standards
- Conjugation of Anti-Rat Antibodies
- Consultant needed
- Contract lab needed
- control diploid used in cell cycle analysis
- cord blood staining
- Core Manager Position-Chicago
- Core Manager's Meeting announcement
- Core Manager/director Survey
- Core Managers benchmark survey request
- Core recharge strategies for new instrumentation
- Correlation between cell viability/cytotoxicity studies
- Costar 50 and 100ml reagent reservoir
- costar reservoirs
- Coulter counter for sale
- Coulter FC500 versus BD FACSCalibur
- Coulter XL - FlowJo question
- Cox-1 & Cox-2
- CPT codes
- CPT coding for CD4/CD8, revisited.
- Creating a database of FCS files
- cryopreservation of lymphocytes for flow analysis
- cumulative apoptosis
- Current protocols in Cytometry
- custom-conjugation of antibodies
- Cy-Chrome-detection
- cy5 antibody
- CyFlow counter from Partec
- Cytometry CDrom Volume 8 - almost last call....
- CYTOMETRY IVth Edition
- DAF-FM diacetate and NO in EC?
- Daily optimization
- Data storage on DVDs
- Daudi Cell Line in mice
- DC linage 2 stage protocol?
- Deadline approaching for FloCyte Advanced Flow Cytometry Course in Madison, WI
- Deadline April 16 for Flow Course in NYC
- Deadline for Adv. Flow Course in Madison
- Deadline for FloCyte Regional Training Program Courses in San Diego - July 21
- Dear all,
- Debris in sample prep
- deciding if 2 pops are the same, and if negative.
- Definition of Near-diploid in breast cases
- Dendritic cell lines...
- Dendritic cells
- Detecting Intracellular IL-10/IL4
- Detecting microchimerism in blood
- Detection of effector from effector memory cells in rat
- Detection of Sepahrose beads by FCM
- detection of smad proteins
- Detection of Viruses on a FACSCalibur?
- Detroit Users Group Meeting 6-18-04
- Dichroic filters
- Dick Hoagland's current email address?
- Difference in Rho-123 fluoresence
- differential cell count - mouse peritoneal lavage cells flow staining
- Digital Channel Number
- digital optical web site
- dilution effect?
- DiOC / PI question
- Disaggregating Needle Biopsy
- Discovery Image Analyser from BD
- Discrimination of GO/G1
- Discussion list summaries
- Discussion of a Human Cytome Project at the E.M.C.
- Display problems with CQ Pro OS X
- Displaying Indo-1 data
- Distance Learning Course
- DIVA
- DIVA 4 auto compensation issue?
- DiVa 4 Summary
- DiVa 4.0 FCS export
- Diva 4.0 files
- DiVa 4.0 Software
- DiVa 4.0 users question
- Diva for sale
- DiVa ND filter holder modification?
- DiVa problems
- DIVA problems? Calcium anyone?
- DiVa v4 problems
- DiVa-FlowJo axis events
- DiVa/Turbo air source?
- DiVa/Turbo help anyone?
- DNA Analysis on Archival Breast Tissue
- DNA cell cycle data Question
- DNA content and DNA index for zoo animals
- DNA content of nuclei from dried plant tissue
- DNA extraction from fixed cells
- DNA extraction from formaldehyde fixed cells
- Dna fragmentation in sperm cells
- DNA ploidy analysis of seed
- Do your have any DIVA problems?
- Does anyone have Dr Carl Stewart's email address? His last one (thank god for the train) bounced.
- Double cytokine staining of cells
- Down regulation of human CD8+
- dr. mr. on Apple
- DRAQ5
- DRAQ5 Source in US?
- DsRed
- DsRed beads?
- East Africa Cytometrysts
- ECFP analysis on Vantage with 457nm line (secondary laserline)
- Ecological question: how to differentiate between species by their pigmentcomposition?
- editing list mode data file
- EDTA solution
- Educational Opportunity
- Effect of time on viability
- Effects of freezing and DMSO on expression
- EGFP Calibration beads and BD LSR-I Machine?
- ELISA on polystyrene microspheres
- ELISA vs CBA
- Employment Opportunity
- Endothelial cells
- Endothelial cells summary
- endothelium
- Eosinophil analysis
- EPICS V Sample Caps
- essential Aria part
- Estimados colegas y amigos de Madrid y Espańa
- ethidium monoazide and annexinV
- evaluating in house conjugated dynal beads
- Evan's Blue Dye
- Excess Flow Cytometer
- Excluding dead cells on phagocytosis assay.
- Experience with G0/G1 in live cells with flow
- Experiences with Cytek Time zero system for Calcium flux
- Expired antibodies
- EXPIRED ANTIBODIES FOR FLOW
- exporting raw data from gated events
- External Proficiency Testing
- Extinction Coefficient of Quamtun dots
- eYFP and 7AAD
- FACASARRAY
- FACS Analyzer to go
- FACS Aria and LSRII Red Laser
- FACS Aria compatible software
- FACS Aria Compensation
- FACS data analysis
- FACS Diva and Windows XP
- FACS DiVa question
- FACS for quantifying surface antigen with MESF
- FACS Pricing advise
- Facs sorter for sale
- Facs sorters email list starting
- FACS staining for phospho-Kit
- FACS Turbo analysis tips?
- FACS Vantage Filter Assembly, FLF* Ultipor ® N66® Membrane
- FACS Vantage Filter Assembly, FLF* Ultipor® N66® Membrane
- FACS Vantage Filter Assembly, FLF™ Ultipor® N66® Membrane
- FACSAria
- FACSAria and calcium flux
- FACSAria Contamination
- FACSAria Optics Upgrade
- FACSAria stream stability
- FACSAria, LSR II, FACSCanto etc... FOLLOW UP COMMENT
- FACSAria, LSR II, FACSCanto etc... looking for user experiences
- FACSCalibur Mistery
- FACSCalibur remodeling
- FACScan power problem
- FACScan sorter?
- Facscan upgrade?
- FACSCanto
- FACSCanto & Bekcman Coulter FC500
- FACScanto...
- FACSConvert for PC???
- FACSDiVa software
- FACSDiVa: application of the same gate to data from several samples
- FACSstar plus dual laser to give away
- FACSstar plus dual laser to give away (incl. location of the instrument)
- FACSTAR PLUS FOR SALE
- FacStar Plus spare parts
- FACSVantage laser
- far red live cell impermeant stain
- fate of irradiated cells in culture; detection by flow
- FC500 files
- FC500 files - parameter name mix up
- Feedback on Cyflow Green Partec
- Feedback on Partec CyFlow ML
- feline basophils
- Fiber optics etc
- fiberblasts anf myoblasts
- field agent job opening
- Field Service Engineer Job Posting
- Filipin staining.
- FISH in suspension
- Fix & Perm delicate cells
- Fix for FACSAria nozzle tip degradation?
- Fixative for Fura-2 (etc)-loaded lymphocytes?
- Fixed and permeabilized Cells and PI
- Fixing Cells, after hour use
- Fixing FITC-annexin/PI-stained cells--need help ASAP
- FL-2 for Flow FISH
- FloCyte Regional Training Program Courses offered in San Diego
- FloCyte Regional Training Program Flow Cytometry Courses in Ft. Worth, TX
- FloCyte Regional Training Program, Basic Flow Cytometry Course in NY City
- FloJo versus Paint-A-Gate
- Flow adhesion studies
- Flow count flourosheres
- Flow Cytometer cleaning
- Flow Cytometrist Positions Open
- flow cytometry
- Flow Cytometry - Research Specialist, Laboratory position
- FLOW CYTOMETRY CHARGES
- Flow Cytometry Course
- Flow Cytometry Courses in 2004
- Flow Cytometry in India
- Flow Cytometry Markers
- Flow Cytometry of Retina cells
- flow cytometry on fat droplets?
- Flow cytometry postion - Seattle, WA
- Flow Cytometry QA
- Flow Cytometry Research Associate at UMass Medical School
- Flow Cytometry Seminar
- Flow Cytometry Supervisor Position in San Diego
- Flow image analysis
- Flow Lab at Cancer Research UK
- Flow List: mouse epithelial cells
- Flow meeting in UK
- Flow on Frozen Tissues
- Flow Phagacytosis Assay
- Flow Phagacytosis Assay Response
- Flow Position at University of Chicago
- Flow related equipment - Sydney garage sale
- Flow Safety Hood
- Flow-Cytometric Controversies
- Flowcytometry
- flowing bacteria
- FlowJo
- FlowJo 6.1 and OSX.5.3 crashing
- FlowJo dongles now secured
- FlowJo seminar schedule - anyone near Detroit interested?
- Flowjo vs Modfit LT
- Fluorescence Intensity
- fluorescence trigger for bacteria
- fluorescent beads phagocytosis
- Fluorescent flow standards, also CD38 flow testing of CD8 + T cells
- Fluorocrome
- FMC7
- FMH
- FMH Testing
- FMO - compensation
- Follow-up to the Fixation Question with FITC-Annexin
- Follow-up to UV laser instrument-Thank You
- FOR SALE
- FOR SALE : BD FACS Vantage SE/TSO
- formaldehyde and autofluorescence
- formaldehyde inactivation
- Free one day course in Practical applications in Cell imaging and analysis
- Freedom of software download from the US
- Freedom of the Scientific Press Under the Bush Administration
- Freedom of the Scientific Press Under the Bush Administration - Followup
- freeware convertion tool: FCS 3.0 to raw channel data ?
- freeze stimulated T cells
- freezing samples
- Friends/Aria users
- further on comparisons (this one's small!)
- Fwd: Another Acridine Orange Question
- Fwd: Cancer Molecular Pathology Training Program at the University of Pennsylvania
- Fwd: DiOC / PI question
- Fwd: mac vs. pc
- Fwd: parameter decriptions fail to show up in WinMidi
- Fwd: scl facs
- Fwd: Single cell sorting with the Aria
- Fwd: viability of sorted cells. SUMMARY
- Gating for apoptosis
- Gating live GFP-positive cells - question
- GFP and Annexin
- Gfp and mitochondria on FACSCalibur
- GFP and YFP together
- GFP Cells + TUNEL Assay (again)
- GFP compensation
- GFP transfection
- GFP transfection, thanks
- GFP-Cy5 compensation
- GLIIFCA deadlines approaching
- goat isotype IgG control
- good anti-GFP antibody for flow
- Good fluors for intracellular
- good marker for mouse spleen macrophage cells
- gradient centrifugation
- Grass skirts and Hawaiian shirts
- Green fluorescent stain for K562 cells
- green laser replacement
- gremlin?
- Groupe d'Utilisateurs Parisiens (GUP)
- GUAVA PCA
- Happy Holidays and a New Year's Proposal
- Heads up for people using WinZip to extract "tar" files.
- Health InterNetwork Access to Research Initiative (HINARI)
- Help with DNA/RNA stain
- help with harvesting cell lines
- Hematopathology Fellowship
- Hemolymph
- Hi Flowers
- Hi vs Low setting?
- High Content Screening (HCS) Website and survey
- High Electronic Mask
- High Grade, Large Cell Lymphomas
- High resolution graphics of flow plots
- High Speed Cell Sorting - FACS Aria or MoFlow?
- His tag antibody
- histogram raw data
- HIT testing
- HL60 (TB)
- HLA DR, DP, DQ Cross reactivity in cats
- HLA-A2.1 typing
- HLA-B27 Control
- HLA-DR staining of MDDCs
- HO342 on FACSVantage
- Hoechst 33342 DNA staining
- Hoechst on Aria
- Holding phagocytosis assays
- HOP26
- How close compensation is good enough?
- How many specimens does it take
- Human lymphocyte culturing
- Human_Cytome_Project
- identifying lymph node macrophages
- IFN far red tandem conjugates & a question for Mario
- IFN far red tandem conjugates: responses
- IFN-g
- IgD testing
- IgVh mutation analysis wanted for a ZAP 70 study
- IL-10 receptor
- Image Flow Cytometry seminar New York!
- Imaging and Microscopy CD-ROM Vol 2 available
- Imaging cytometry seminar at NIH
- Immediate opening: Cytometry position at University of New Mexico, Albuquerque
- Immufuge II heads
- Immuno meeting London UK
- immunocytochemistry
- Immunoflourescent Staining Help Needed
- Immunofluorescent Staining Help Needed
- immunoglobulin
- Immunology grading
- Important Workshop Announcement
- In vitro Micronucleus Scoring via Flow
- In vivo cell tracking
- Inactivating FCS
- Indirect intracellular staining of whole blood
- Indy Flow Users Meeting Jan 12
- Innova-90 laser
- installation qualification certificate
- Interface to an LIS
- International CD4 Counting Multicenter Study
- Intracellular Cytokine - Optimal time
- Intracellular Cytokine Staining
- Intracellular cytokine staining vs. ELISPOT: some answers
- intracellular staining of ... 2004
- Intracellular TNF
- intracellullar staining
- intranuclear staining
- intranuclear staining for flowcytometry
- Introduction to Histograms
- Invalid FCS files from Cellquest
- Invitation to Contribute to Imaging and Microscopy CD-ROM
- Invitation to ISAC Workshop: Cell Sorting for Microgenomics
- Iranian Journal of Immunology
- Is fixation needed for IC staining? Detecting F-cells
- Is It possible to do GFP and YFP together
- ISAC - MultiColor Flow Cytometry
- ISAC abstract contact
- ISAC Compensation Workshop
- ISAC Congress Workshop "The Cytometry of Plants"
- ISAC Core Managers' Workshop
- ISAC Montpellier CyberCafe
- ISAC Montpellier Workshop: "Light Scattering Revisited"
- ISAC Workshop Handouts
- ISAC Workshop on DNA and Solid Tumors
- ISAC Workshop: Light Scattering Revisited
- ISAC XXII - reminders
- ISAC XXII - some things to attend perhaps
- ISAC XXII progress - Day by day account!!!
- isolation mouse PBMC
- Isolation of granulocytes in Chediak Higashi Syndrome or in Specific Granulocyte deficiency
- Java Error Message with DiVa software
- JC-1
- JC-1 and fixation
- JC-1 fixation
- Job Opening
- Job Opening: Field Service Engineer, Pacific Northwest Region
- Job Opportunities
- Job Opportunity
- Job Opportunity (Canberra, Australia)
- Job Opportunity - Flow Cytometry Technician
- Job posting -- RA II/Flow Cytometrist, KineMed, Emeryville, CA
- Job posting: Boston MA
- June 15 DEADLINE for FloCyte Regional Training Program Flow Cytometry Courses in Ft. Worth, TX
- Kappa / Lambda
- kenyanflowcytometry.4t.com
- labeled siRNA in combination with EGFP
- labelling LAMIN B1 analysis by flow cytometry
- Lack of CD4 cells in a patient with Waldenstrom's Macroglobulinem ia
- Lack of mail today (Wednesday)
- Lambda/Kappa
- LAMIN A/C detected by FACS
- LANL Laser Incident
- Large nozzle sorting on BD machines
- large particle analysis and sorting
- large particle analysis and sorting-thanks
- Laser Replacement Options from my dead FACScan
- Laser safety eyewear (generally and specifically for Spectra Physics Millennia IIs)
- Laser saftey eyewear (generally and specifically for Spectra Physics Millenia IIs)
- Laser Scanning Cytometer
- leucocyte fixation
- level of expression between 2 cell populations (a theoretical question)
- Levey-Jennings
- liboarabinomannan specific mabs
- little correction
- Live cell marker?
- Live or dead cell marker
- Login accounting on Mac OS 10
- Long wavelength dyes for in vivo staining?
- looking for a new challenge
- looking for Anna Porwit
- Looking for Flowcytometry trained personnel
- looking for new employment
- loss of all induce cell surface marker expression
- Loss of erythroid precursors from bone marrow during Whole Bl ood Lysis technic
- Loss of erythroid precursors from bone marrow during Whole Blood Lysis technic
- loss of fluorescence intensity
- low antigen-specific CD4 responses from frozen PBMC
- low purity sorting
- Low yeild after sort
- Low yield after sort
- LSC- ploidy on tissue sections
- LSR II Configurations
- LSR II red Trigon Filters
- Lucifer yellow alternative
- luminex beads
- Luminex For Sale
- Ly-6G (Gr-1) expression on mouse monocytes
- Lymphocyte Apoptosis Induction
- Lymphocyte subset enumeration phenotyping
- Lysosomal tracker together with intracellular antigen
- M ean Channel Fluorescence
- mac vs PC
- mac vs. pc
- MacAdministrator 3.1
- Macroscopic GFP visualization
- Magnolia Ploidy and chromosomes
- malarial parasites protocol
- marker to differentiate monocytes vs. pDCs
- Markers expressed on fat cells
- Markers for Fibroblasts
- MASIR 2005 Conference - Courmayeur, Italy - January 26-30
- MASIR 2005 Conference - First Announcement!
- maximizing recovery of paraformaldehyde-fixed cells
- Maximum suggested Compensation on a Calibur
- MDDCs
- measuring 488 in UV
- Measuring cell cycle and apoptosis simultaneously
- measuring viability in intracellular staining
- Medarex antibodies
- meeting
- MEETING ANNOUNCEMENT
- Meeting of the Clinical Cytometry Society - Announcement
- MEETING REMINDER
- Meeting Reminder: Boston Users Group / CFAR HIV meeting
- Mef 2c GATA4 Nkx2.5 miocardial transcription factors
- Melles Griot 35mW HeNe laser
- Membrane dye for living cells
- Memory T- cell marker.
- Memory T-cells
- Merging data files
- MetroFlow meeting announcement
- MetroFlow Spring Meeting
- MF
- MFI meaning
- MFI vs % positives - specific to CD4+CD25bright
- MFI vs %Positive: myths deserve answers
- MHC Class I Pentamers
- MHC tetramer/CD8 antibody
- microglia
- microparticles
- Minimal volume to justify stand-alone cytometry laboratory
- Minimium amount of GFP molecules for detection
- MIP 1 alfa by flow
- Missing DiVa display data
- mitochondrial mass
- Mitomycin and Secretion of Cytokines
- MoAb for anti mouse MDR1
- MoFlo
- MoFLo and GMP
- MoFlo's
- Molecular Probes Live/Dead Kit
- Monitoring users access
- Monkey Pgp MAb and efflux substrate
- More Credit for Comp Workshop Video
- More MoFlo stuff
- More parts
- Most valuable Cytometry (Journal) papers - Send 'em in!
- Mouse dendritic cell characterization
- Mouse Intracellular IFNG
- mouse peripheral lymphoid cell sorting
- mr on Apple web site
- mr on Apple website
- MRK-16 with PE or FITC or ...
- MultiColour Workshop, 1 December,Boston
- Multiparameter Flow and Compensation course at Madison WI
- multiple color enquiry
- murine fibroblasts
- Murine macrophages and dendritic cells
- myeloperoxidase
- N2 Assay
- NAD(P)H autofluorescence
- NCCLS quantitative flow std - clinical labs
- need advice on configuring a microscope for live cell imaging
- Need help finding antibodies directly conjugate to dyes for a violet laser.
- need help quick!
- Need of a 7D6 antibody
- neutrophil elastase
- neutrophil oxidative burst
- New "Purple top" K3 EDTA tubes
- New article - HLA-DR regulation and the influence of GM-CSF on transcription, surface expression and shedding
- New CPT codes
- New Flow Cytometry Reimbursement
- New flow user
- New flow/image instrument
- New Purdue CDROMs
- New QA test for spectral confocal microscopes.
- New York (Western/Upstate) regional flow users group forming
- Next ISAC Congress?
- NF-kB Staining
- NK cells viability post sorting on FACSAria
- No more sticky cells.
- Nocodazole and mimosine
- Nonreadable FCS 3 files from Coulter FC 500 cytometer
- odd leukocyte FSC vs. SSC
- offline analysis
- OKT8 antibody
- Old Parts for Training
- OmniComp needed
- once again, comparisons...
- One Lambda HLA antibodies
- Open meeting @ ISAC XXII: Bioinformatics Standards for Flow Cytometry
- operator position
- Operator Position @ Gladstone Institutes, SF, repost
- Optimal DNA histograms from wax sections
- optimal flow cell size for ecoli?
- optimum concentration for sorting
- Optiprep reduction of contaminating platelets
- Organizational meeting for a Portland area user's group
- OS X???
- OSX login software
- Overlay on FACSDiVa
- Overstaining question
- Overstaining summary
- P antigen
- P-PACK tubes
- p38 and MAPKAPK-2 staining in whole mouse blood
- Pam Shaw >off line anaylsis /data backup
- parameter decriptions fail to show up in WinMidi
- PARP and PAR immunoglobulin and protocols for FACS
- Partec's CyFLowML
- Parts to offer to the cytometry web group
- PBMC Stimulation
- PC Login Tracking Software
- PE-Alexa Fluor 610 (Caltag)
- PE-Cy7 stability
- PE/CY5 fluorescence problem
- PerCP vs PerCPCy5.5 (or PE-Cy5.5) on FACS Aria or LSR II
- PerCP-Cy5.5 or PE-Cy5?
- PerdueCytometry: Fluoro Gold viability stain
- persistence of labeled mAbs in week long cultures
- PHA not working
- Phagocytosis
- Pharma/Biotech Cytometry Group
- Phenotyping of Osteoblast / Osteoclasts
- Phospho-Histone H3 and Active caspase
- phospho-STAT4
- Phosphoprotein staining
- PI
- PI and cell lines
- PI at 488?
- PI cell viability test
- PI concentration
- PI Staining
- PI staining of chicken embryo fibroblasts
- PI/ Annexin V staining
- plantDNA
- Plasma Cells & MM
- Plasma cells and CD45
- platelet
- platelet aggregometer
- Platelet autoantibodies
- Platelet concentrates
- Platelet free Leukocytes
- Platelet Microparticles
- Platelet-free leucocytes
- Platelets and cold-induced aggregation
- Ploidy Analysis
- PMT or log amp linearity? (calibration beads)
- politicalization of science
- polylysine coated slide concentration
- Population comparison for publications
- Porcine leukocytes
- Position at Hackensack-flow cytometry
- Position available
- Position available at the NIH
- Position Available: Director, National Flow Cytometry Resource
- Position in San Diego
- Positive control for Tissue Factor
- Positive control for Tissue Factor-Summarized
- post-analysis software question FCS 2.0 and FCS 3.0
- post-analysis software question FCS 2.0 and FCS 3.0 [2]
- Post-doc position in flow cytometry available at Los Alamos National Laboratory
- Post-Doctoral Position Malaria
- Postdoc at Purdue University
- Power consuption FACSAria vs FACSVantage
- Preliminary Announcement - Western NY Flow Users Group Forming
- Prionex
- Problem with calcium flux data from DiVa in Flowjo
- problems with ACK/RBC lysis buffer
- problems with ACK/RBC lysis buffer again
- Problems with ammonium chloride lysing
- Problems with CFSE assay, RBCs?
- proficiency testing
- Proliferation analysis with FlowJo
- protein redistribution (cytosol or nucleus)
- Pulse Processing on the Vantage
- Qdot streptavidin conjugate and secondary antibody
- Qdots
- quality control for CD4 enumeration
- Quantification of signals when dealing with auto-fluorescence
- Quantification of virus inside cells
- Quantification of virus inside the cells :responses
- quantitating GFP gene expression
- Quantitation in FCM
- Quantitative Flow Workshop at ISAC: "Flow by the Numbers"
- quantitative protein stain
- Question
- question about cytokine beads array
- Question about FACS on primary human solid tumour cells
- question about MFI in flowjo analysis
- Question about new CPT codes
- question concerning intracellular FACS
- question concerning use of immunohistochemistry antibodies for fl ow
- question for bacteria
- question for Luminex users
- question on glucose transporters
- Question on Howard's book
- RA (II) flow cytometry specialist position at SF Bay Area biotech startup company
- rabbit lymphocyte markers
- Rant I had
- Rare event sorting....
- Rat haematopoetic stem cells?
- rat neut's mono's
- Rat rbc lysing
- ratios or absolute values?
- RBC associated IgG
- rbc lysis vs retics
- RE : FACSConvert for PC???
- RE : New flow/image instrument
- RE : RE Aria ideal max. sort rate w/ viable cells
- RE Aria ideal max. sort rate w/ viable cells
- re CD34
- re exporting raw data
- re mac vs pc debate
- re nonreadable FCS 3 files
- Re.Live/Dead-Assay
- Recent messages again
- Recent political messages
- Receptor capping
- Red Cell Membrane Abnormalities
- Reference beads
- Reference regarding PI
- regarding question use of immunohistochemistry antibodies for Flo w
- Registration deadline Monday Sept 20 for Adv. flow Courses in NYC
- reimbursement
- Reminder - Imaging cytometry seminar at NIH
- Reminder - Seminar announcement and Satellite BUG Meeting
- Reminder- Upcoming Chesapeake Cytometry Consortium Fall Meeting
- Reminder: EWGCCA EuroConference, Mol, Belgium
- Reminder: Vendor Support for the Boston Users Group for Cytometry / CFAR HIV Meeting
- Reminder: Western NY Flow Users Group Registration Deadline comi ng up
- Replacing a FACSVantage...is a good choice?
- Reply: anti Pichia antibodies
- Reply: Medarex antibodies
- Reporter Genes and MFI
- Representing changes in expression levels
- request for protocol-regarding
- Request for User Group Resources
- research scientist position.
- Research Triangle Cytometry Association 10-November-2004 09:00 EDST GSK RTP
- Research Triangle Cytometry Association 12-Aug-2004, 9:00 AM Eastern, Eli Lilly, RTP Laboratories
- Research Triangle Cytometry Association 17 March 10:00 AM NIEHS
- Research Triangle Cytometry Association 20 January 09:00 AM
- Research Triangle Cytometry Association 22-Jun-2004 09:00 US EPA
- Research Triangle Cytometry Association 28-Oct_2004 09:30 WFU Baptist Med Center
- Research Triangle Cytometry Association 29-Sep-2004, 09:00, GlaxoSmithKline
- Research Triangle Cytometry Association, 05-May-2004, Duke Univ, 10:00 Eastern
- Residual charges in the stream ?
- Resource Mgr's Workshop at ASH?
- Responses to BD CompBeads question!
- responses to gfp/yfp question
- responses to questions
- responses to questions: THIS IS WHAT WE ARE DOING!!
- Review of manuscripts with digital acquired data
- RFI's and so forth
- RFI's on longitudinal data (question restated)
- RNA extraction and cell sorting
- RNA from fixed and sorted cells
- ROS detection questions
- ROS production - positive control
- Réf. : Annexin staining with platelets
- Réf. : Cell Counter
- Réf. : FACS for quantifying surface antigen with MESF
- Réf. : Fluorescence Intensity
- Réf. : Plasma cells and CD45
- Réf. : platelet
- Réf. : Quantification of signals when dealing with auto-fluorescence
- S phase?/WinMDI
- S-P Vanguard 355 nm laser
- salaries for flow cytometry operators versus supervisors
- Salary
- same-antigen on the surface and intracellularly
- Sample flow rate
- Sample Prep and Lyse Wash Assistant for BD FACSCalibur
- Sample Prep and Lyse Wash Assistant for BD FACSCalibur -Reply
- SAN DIEGO AREA FLOW CYTOMETRY USERS' GROUP
- SAN DIEGO AREA USERS' GROUP
- San Diego Cytometry Position -- Re-post
- Scatterplot attachment for AUL
- ScFv labeling protocol
- SEB activation of CD4+ cells
- Secondary antibody with PE not binding to anti - CK20
- securing USB dongles
- Security and tracking software for XP
- Seeking flow lab in Northwest
- Seeking new position.
- Seeking your replies (was: Antwort: Gating for apoptosis)
- selective loss of CD8+ cells during ficol
- Seminar announcement and Satellite BUG Meeting
- Senior Biologist Position for biomarker assay research
- Setting compensation level using cellquest+FACScan
- setting up 6 color sort; questions
- setting up FSC/SSc
- Sheath Fluid
- sheep antibodies
- Siberian Hamsters
- siGLOsiRNA
- SImple question
- Simultaneous intracellular stain and DNA Prep
- SIMULTANEOUS SURFACE AND PI STAINING QUESTION
- Single cell sorting with the Aria
- siRNA-Cy5 tranfected PBMC
- size-different particle with same fluorescent intensity
- small correction to previous post.
- small things
- SNARF-1 labeling
- sodium azide
- sodium azide concentration in reagents
- Software recommendations for offline analysis in PC-land
- Solid State Laser replacemnt for FACScan/Caliber
- Someone is looking for employment
- sorry-one more question...and a rant
- Sort mouse brain cells
- sort mouse NSCs
- Sorter Hours Accounting Summary
- Sorter location.
- Sorter operator position
- Sorting - LS threshold?
- Sorting Bacteria
- Sorting based on estrogen receptor
- Sorting by Chemiluminescence
- sorting cells for elisa
- Sorting conditions for NK cells - Resume
- sorting dim cells
- sorting dim cells, Barbara Taylor
- Sorting EGFP
- sorting EGFP transfectants
- sorting fixing cells for RNA extraction after sorting
- Sorting for rare cell RNA
- sorting GFP transfected cells treated with RNALater
- sorting in the Baton Rouge area
- Sorting isolated mitochondria
- Sorting issues
- Sorting kills cells?
- Sorting Live Cells for Chromosomal Content?
- Sorting low number of cells
- Sorting of E. coli cells and DNA content analysis on BD FACSCalibur
- Sorting of EBV transformed Human B cells
- sorting retics
- sorting yeast on a moflo
- Sorvall or Helmer Cell Washer
- source for good secondary antibodies conjugates
- source of F11 cell ?
- SPA and Lyse/Wash from BD
- SPA and Lyse/Wash from BD -Reply
- Spare parts for MoFlo systems
- Specific marker of vascular smooth muscle cells
- spectra text files repository
- Spectroscopic Imaging-Validation
- spectrum of" cell tracker orange"
- Spermatogonia sorting with FACS
- ssc/CD45 mouse bone marrow
- staffing issue
- staining nuclei with DAPI for sorting
- Staining of murine cells for HSC
- Stations Automated vs Manual
- STATISTIC QUESTION (RFI'S)
- stem cell applicatins by using FACSCalibure flowcytometere
- stem cell from human cord blood
- stem cell research and flow cytometry
- sterile sorting
- Sterile sorting - summary
- Sterile sorting - Summary (one more)
- Sticky cells
- suggestions to calibrate FACScan
- Summaries!
- Summary - Sorting Kills
- Summary - too bright eGFP
- Summary - Viable by LS; dead by PI (?)
- Summary of 96-well plate autosampler responses
- Summary of replies: Question for Luminex users
- Summary of responses - Fix & Perm delicate cells
- Summary of responses: CD3-CD4+CD14-
- summary of:What version of OS9 works best w/Facscan/G3 combo
- Summary on 405 vs 532nm laser
- summary securing USB dongles
- Summary/FACS Vantage air compressors
- Summary: Coulter XL - FlowJo question
- Summary: ECFP analysis on Vantage with 457nm line (secondary laserline)
- Summary: immunocytochemistry
- SUMMARY: RFI'S ON LOGITUDINAL DATA
- Summary: SNARF-1 labeling
- Summery of responses for question "Loss of erythroid precursors from bone marrow during Whole Blood Lysis technic
- Super Cores???
- surface and cytoplasmic antigen staining
- surface CD22 in EGIL score
- surface marker for epithel-fibroblasts
- Surplus equipment
- Surplus laser equipment for NIH intramural labs
- suspension arrays
- SV: Adult peripheral blood
- SV: Antibody titrations (2004...2005...2006... and beyond)
- SV: Any suggestion for selection of ICAM-1 (CD54) antibody?
- SV: marker to differentiate monocytes vs. pDCs
- SV: Markers for Fibroblasts
- SV: why are anti-human mAb concentrations omitted?
- Svar: Réf. : Cell Counter
- SYBR in intact cells
- T cell activation assay
- T cell expressing different CD3 in PBMC
- Tax antibody
- Telomere length studies
- Temperature control for sample tubes
- Temperature control for sample tubes - summary of replies
- TetraTag™ PE-Labeling Kit
- Th-1 and Th-2 tracking in Cynomolgus monkeys
- Th-1 and Th-2 tracking in Cynomologus monkeys
- TH1/TH2 cytokines with CBA
- Thank you
- Thank you for your help
- Thank you!
- Thanks all for helpful comments about PI cell viability check
- Thanks for the DIVA tip
- Thanks from Madrid-Spain
- The Clinical Cytometry Society Cyber Café
- The sound of sorting
- Thiol levels
- Thymidine block and PI staining
- TLR5
- TNFRI staining
- To all those in shock and sadness about the tragedy in Spain
- To-Pro 3
- too bright eGFP
- total fluorescence and cell aggregates
- TQ-prep vs BD Lyse/Wash assistant
- Tracker dyes with Fluo-3/FuraRed Measurement
- tracking cell division
- Transfection siARN-Cy5
- TRAP 6
- Tricky nuclear staining
- tubing question
- Tunel
- TUNEL staining
- TUNEL vs Annexin V
- tunel with adherent cells
- Turning a cytometer into a plate reader ;-)
- Two photon laser flow cytometry
- UK Cell sorter for sale!
- Uncertainty of Measurement
- University of Maryland, Baltimore - Position Opportunity
- University of Missouri-Columbia --job opportunity
- Unknown profile with 7aad
- unspecific fluorescence in detached cells
- Upcoming Chesapeake Cytometry Consortium Fall Meeting
- Upcoming Chesapeake Cytometry Consortium meeting
- Upcoming ChUG Seminar
- Update on ISAC 2006 and important info
- Update to Specialty Training & Education Program in Cytometry
- Upper Midwest Flow Cytometry Users Group
- Uptake into macrophages
- Uptake of antigen by dendritic cells
- US reverses journal embargo
- Use of barcode reader on Calibur
- Used FACScan for Sale
- used MoFlos
- User Group
- Using SNARF-1 with eGFP
- UV excitable dyes
- UV Laser -- Who's selling?
- UV laser instrument
- UV Lasers
- Vacuum pump recommendations
- VCAM-Ig fusion protein
- Vendor for laser safety glasses?
- Vent Kit for a FACScan
- verapamil and SP population
- Vertical motion timeouts
- Vertical Motion timeouts on Facscalibur
- Vesicles
- Viability of sorted cells
- Viability using 7-AAD
- viability-check after SNARF-1
- viable by LS but dead by PI?
- vibration and optical precision
- Vimentin Staining of Fibroblasts by flow
- visiting Immunology research labs in Canada
- Volume of cells
- Wanted - FSP pressure tanks
- Wanted: FACScan Overhaul
- wanted: old epics elite whole or parts
- Wanting to buy a Sorter, is necessary?!
- Waste containment on benchtop analyzers
- water pressure problems for Laser
- Water re-circulators
- We are thinking of buying two new bench top flow cytometer (2 CYANs or a LSRII/FACScanto).
- What other floresence can I use with FITC, PE and Propidium Iodide ?
- What value to use in a population comparison
- What version of OS9 works best w/Facscan/G3 combo
- where can I buy CD85j ab
- Where is the windows version of Ldata
- which conjugates can be frozen?
- Whole Cell FISH
- Whole liver mouse hepatocyte isolation
- why are anti-human mAb concentrations omitted?
- Win MDI Stats
- WinMDI renaming axes
- WinMDI upgrade for Windows XP?
- WINMDI2.8 and LInux
- WNY Flow Users group
- WNY Flow Users group Call for Abstracts
- WNY-FUG 4 weeks and counting
- Workshop on Hematologic Controversies
- Workshop: Advance Education and training in Cytometry
- Y chromosome specific probe for flow?
- yeast cell synchronization
- Zap 70
- Zap-70
- ZAP-70 and CD38 revisited
- zap-70 antibodies
- ZAP-70 in CLL: Is it reliable ?
- ZAP-70 vs. CLL
- Zebrafish Embryo Sorting
- Zenon for intracellular labelling
- Zenon labeling reagent
- zenon labels vs fab conjugates
- Zn-chelating buffers
- Zymography on paraffine sections
- Last message date: Wed Dec 29 2004 - 18:38:00 EST
- Archived on: Mon Jan 03 2005 - 03:12:07 EST