Re: Overstaining question

Andy,

Perhaps what your source was referring to is the "prozone effect" which 
occurs in agglutination reactions.  I'm not certain that it applies to cell 
surface antigen staining; however, I have used it as a "possible 
explanation" for results that could also have been caused by someone 
forgetting to add a reagent/mAb.

See the following:


1) prozone

<<http://cancerweb.ncl.ac.uk/cgi-bin//omd/contents/immunology.html>immunology> 
Prozone phenomena occur in 
<http://cancerweb.ncl.ac.uk/cgi-bin//cgi-bin/omd?immunological>immunological 
<http://cancerweb.ncl.ac.uk/cgi-bin//cgi-bin/omd?reactions>reactions when 
the 
<http://cancerweb.ncl.ac.uk/cgi-bin//cgi-bin/omd?concentrations>concentrations 
of <http://cancerweb.ncl.ac.uk/cgi-bin//cgi-bin/omd?antibody>antibody or 
other <http://cancerweb.ncl.ac.uk/cgi-bin//cgi-bin/omd?active>active 
<http://cancerweb.ncl.ac.uk/cgi-bin//cgi-bin/omd?immune>immune 
<http://cancerweb.ncl.ac.uk/cgi-bin//cgi-bin/omd?agent>agent are so 
<http://cancerweb.ncl.ac.uk/cgi-bin//cgi-bin/omd?high>high that the 
<http://cancerweb.ncl.ac.uk/cgi-bin//cgi-bin/omd?optimum>optimum 
concentration for 
<http://cancerweb.ncl.ac.uk/cgi-bin//cgi-bin/omd?maximal>maximal reaction 
with <http://cancerweb.ncl.ac.uk/cgi-bin//cgi-bin/omd?antigen>antigen is 
exceeded. Immunological phenomena in the prozone 
<http://cancerweb.ncl.ac.uk/cgi-bin//cgi-bin/omd?region>region may 
<http://cancerweb.ncl.ac.uk/cgi-bin//cgi-bin/omd?show>show 
<http://cancerweb.ncl.ac.uk/cgi-bin//cgi-bin/omd?partial>partial or 
<http://cancerweb.ncl.ac.uk/cgi-bin//cgi-bin/omd?total>total 
<http://cancerweb.ncl.ac.uk/cgi-bin//cgi-bin/omd?inhibition>inhibition.

(18 Nov 1997)
http://cancerweb.ncl.ac.uk/cgi-bin/omd?query=prozone&action=Search+OMD

2)   http://www.med.sc.edu:85/mayer/ab-ag-rx.htm

Rick

At 02:57 PM 12/13/2004, you wrote:

>Hi All,
>I was given some information by someone that I find hard to believe even 
>though I think my source is reliable.
>I was told that it might be possible to "overstain" cells causing them to 
>lose their fluorescence.  This was specifically for a CD4 antibody.
>Can anyone confirm or deny this information?
>
>Thanks in advance!
>Andy
>
>
>
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