RE: Rare event sorting....

Hello Christopher,

We routinely do single cell and rare cell sorting in our labs. The
easiest procedure to follow to identify what you have recovered, is to
perform single-cell PCR analysis. Perform a comparative PCR analysis on
a larger sample of the cells of interest if possible, alongside the
single-cell PCR. We have also performed single cell DNA sequencing to
corroborate the PCR findings.

Lisa Reece, BS
Asst. Director, Molecular Cytometry Unit
University of Texas Medical Branch
Dept. of Internal Med./Infectious Dis. Div.
301 University Blvd., Rt. 0435
Galveston, TX 77555-0435
Office 409.747.1927/Fax 409.772.6527
Labs 409.747.1852/Pager 409.643.3812
www.utmb.edu/stem 

"A problem is only a solution waiting to be discovered."

-----Original Message-----
From: Christopher A. Worth [mailto:caw@bcc.louisville.edu] 
Sent: Thursday, December 09, 2004 8:49 AM
To: cyto-inbox
Subject: Rare event sorting....


I've got a question.  I've got some folks that are needing to sort out
small populations.
 .1% or so and smaller.  

How do people prove to themselves that what they've got coming out is
what they want when
you're looking at a sorted quantity of 500 cells or so?  At least 
that's what they expect to get in some cases.

I'd like to be sure we're actually doing what we think we're doing.


Thanks,
Chris