In our hands, the majority of polychromatic and orthochromic erythroid precursors are indeed lost from bone marrow that has undergone lysing using commercially prepared lysing buffers. For enumeration, proerythroblasts and basophillic erythroblasts make it through the procedure relatively unscathed. Renold Capocasale Research Scientist Experimental Pathology, Toxicology and Investigational Pharmacology Centocor, Inc a Division of Johnson & Johnson Inc Ph # 610-651-6421 Fax 610-651-7363 -----Original Message----- From: Bakul Dalal [Dr.] [VH] [mailto:bdalal@vanhosp.bc.ca] Sent: Thursday, December 02, 2004 6:01 PM To: cyto-inbox Subject: Loss of erythroid precursors from bone marrow during Whole Blood Lysis technic We are trying to use flow cytometry to do bone marrow differential count. Does any one know if erythroid precursors are lost during Whole Blood Lysis technic for flow cytometry? Thanks. - Bak Bakul I. Dalal MD FRCPC FCAP FASCP Director, Flow Cytometry Laboratory Assoc Director, Hematology Laboratory Vancouver General Hospital Clinical Professor, Faculty of Medicine University of British Columbia bdalal@vanhosp.bc.ca 604 875 4496 / 4798(f) Life is not measured by the number of breaths we take, but by the moments that take our breath away.Received on Mon Dec 6 17:18:00 2004
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