PBMC Stimulation

Dear Flowers,


I am looking for the cleanest way to stimulate PBMC.  I would like to avoid
ficoll because I do not want loose rare cell populations.  I would also like
to avoid anything that would prevent phenotyping with CD3, 4, 8, 45RA, 62L
etc...


Thanks,  


David A. Bruno, MD

Research Fellow

National Institutes of Health

NIDDK/ Transplantation Branch

CRC 5-5832

301-451-3347