Hi there, Just a quick question. Our group has been doing 'compensation' with FMOs (Flourescence Minus One). I have been saying that we really need single positive controls to do the compensation accurately, but, because we are looking for very rare cells, my boss has argued that they want to take into account all the color interactions when compensating. It is very difficult (for me) to do compensation this way when I have been use to using single colors. What do others think? We are looking for a population that is .01 % of the entire population and we are using 4 colors - Fitc, PE, PerCP and APC on a two laser Calibur. Thanks so much for your responses! Cheers, Kim Lowe Childrens Hospital Boston, MAReceived on Fri Dec 3 13:38:00 2004
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