RE: PI Staining

For a single dye, FL2 or FL3 give a similar readout. Using a double staining
like Annexin I prefer FL3 to reduce compensation. 
You can also use 7AAD replacing PI, reading cells on FL3 for BD FACSCalibur.
Regards Paolo Cappella
Flow Cytometry Unit 
Biology Dept. - Bdg.75 
Nerviano Medical Science srl 
Via Pasteaur 10 - I 20014 Nerviano 
tel. +390331581367 

-----Original Message-----
From: Peter Leposo [mailto:pleposo@inbox.lv]
Sent: Monday, November 29, 2004 2:54 PM
To: cyto-inbox
Subject: PI Staining


Hi Flowers,



Need Help on the above topic.I tried following previous threads but still
have 

unanswered qs.I want to run assays to count live/dead cells with PI as a
single 

dye and i use a calibur with 488 argon laser,where exactly should i expect
the 

signal on the FL2/3 channel? Are there any concentration issues i should 

consider before running my assays?



Peter Leposo

Immunology Laboratory

KEMRI

www.kemri.or g 



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