Re: Sheath Fluid

Adam,

	PBS does not break down at high sorting pressure, and has always 
worked pretty well as sheath for most of the lymphoid sorts I have been 
involved with over the years. However, some cells do seem to come through 
the process much better if the sample buffer has additional buffering 
capacity, and it is not uncommon to see successful labs use 10 - 25 mM 
HEPES added to the sample buffer (usually PBS or Hank's) in addition to 
reasonable amounts of protein. 

	Joe





"Adam Palazzo" <Adam.Palazzo@UTSouthwestern.edu>
11/19/2004 12:43 PM
	To:	Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>
	cc:	(bcc: Joe Trotter/SDCA/BDX)
	Subject:	Sheath Fluid


Recently my BD field application rep came out to help me with my Aria. 
He said that PBS can breakdown with high pressure sorting and that we
should use a normalized saline as our sheath fluid instead.  Does anyone
else use this and can you supply me with the company and the catalog
number of the product.		 Thanks.

Adam Palazzo
Perlingeiro Lab
Center for Developmental Biology
University of Texas Southwestern Medical Center at Dallas
214.648.7354




-----------------------------------------
*******************************************************************
IMPORTANT MESSAGE FOR RECIPIENTS IN THE U.S.A.:  This message may
constitute an advertisement of a BD group's products or services  or a
solicitation of interest in them. If this is such a message and you would
like to opt out of receiving future advertisements or solicitations from
this BD group, please forward this e-mail to optoutbygroup@bd.com.
*******************************************************************  This
message (which includes any attachments) is intended only for the
designated recipient(s).  It may contain confidential or proprietary
information and may be subject to the attorney-client  privilege or other
confidentiality protections.  If you are not a designated recipient, you
may not review, use, copy or distribute this message. If you receive this
in error, please notify the sender by reply e-mail and delete this message.
Thank you.
*******************************************************************
Corporate Headquarters Mailing Address: BD (Becton, Dickinson and Company)
1 Becton Drive Franklin Lakes, NJ 07417 U.S.A.
*******************************************************************