Adam, This is absurd! These is no scientific evidence that sorting under high pressure changes the buffering capability of PBS. Many cell functions studies currently underway on memory cell populations, sorted under high pressure demonstrate no ill effects of the sheath media or the pressure placed on these cell populations. If you are referring to gas solubility, this simply does not happen, at least not to the extent that PH would be altered to damage the sorted cell populations. Hence, while PBS is certainly not an "ideal" sorting media and one would never culture cells using PBS, it does work as a transient media for sorting. SP ======================================================= Stephen P. Perfetto, MS.,MT. (ASCP) Manager, Core Flow Cytometry Facility Vaccine Research Center, NIH Building 40 40 Convent Dr., Room 5507 Bethesda, MD 20892-3015 email: sperfetto@nih.gov Phone: (301) 594-8659 Disclaimer: The information in this e-mail and any of its attachments is confidential and may contain sensitive information. It should not be used by anyone who is not the original intended recipient. If you have received this e-mail in error please inform the sender and delete it from your mailbox or any other storage devices. The National Institute of Allergy and Infectious Diseases (NIAID) shall not accept liability for any statement made that are the sender's own and not expressly made on behalf of the NIAID by one of its representatives. > ---------- > From: Adam Palazzo > Sent: Friday, November 19, 2004 3:43 PM > To: Cytometry Mailing List > Subject: Sheath Fluid > > Recently my BD field application rep came out to help me with my Aria. > He said that PBS can breakdown with high pressure sorting and that we > should use a normalized saline as our sheath fluid instead. Does anyone > else use this and can you supply me with the company and the catalog > number of the product. Thanks. > > Adam Palazzo > Perlingeiro Lab > Center for Developmental Biology > University of Texas Southwestern Medical Center at Dallas > 214.648.7354 > >Received on Tue Nov 23 15:58:00 2004
This archive was generated by hypermail 2.1.8 : Wed Nov 24 2004 - 03:12:05 EST
