Hi Adam: I am running an aria as well. I use a home-made 10 mM Hepes buffered Saline pH 7.2-7.4 for all my sheath fluid and it works great. In my hands, I have had no problems (as measured by viability and growth) on all cells and cell lines (human, mouse, cell lines), populations (HSC, B and T cells, precursor Eyrthoid/myeloid cells, etc), and sorting conditions (2-way, 4-way, single cell sorting and sorting directly into methylcellulose), Hope this helps. Tim Timothy Bushnell, Ph.D. CHGMPD Flow Lab University of Rochester tel: 585-273-1361 http://www.urmc.rochester.edu/wnyfug/ -----Original Message----- From: Adam Palazzo [mailto:Adam.Palazzo@UTSouthwestern.edu] Sent: Friday, November 19, 2004 3:43 PM To: cyto-inbox Subject: Sheath Fluid Recently my BD field application rep came out to help me with my Aria. He said that PBS can breakdown with high pressure sorting and that we should use a normalized saline as our sheath fluid instead. Does anyone else use this and can you supply me with the company and the catalog number of the product. Thanks. Adam Palazzo Perlingeiro Lab Center for Developmental Biology University of Texas Southwestern Medical Center at Dallas 214.648.7354Received on Tue Nov 23 14:18:00 2004
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