Samuele, To the best of my knowledge, the sample pressure is increased for the first few seconds to "boost" the sample to the flow cell. This shortens the time it takes for the sample to reach the flow cell. As far as I know, Cellquest is not lagging behind, and then catching up. It is accurately reflecting the actual number of cells going through the flow cell. In other words, your Facscalibur is working correctly. All the best, Don ++++++++++++++++++++ Donald Smith Cytek Development dsmith@cytekdev.com (510)657-0102 Office (510)657-0151 Fax (925)785-5824 Mobile (no Voicemail!) (925)785-0042 Voicemail only -----Original Message----- From: Samuele Secchiero [mailto:strikeiron13@yahoo.com] Sent: Tuesday, November 16, 2004 2:23 AM To: cyto-inbox Subject: CellQuest problem Hi to all, I'm using a Facscalibur at the moment and I have a technical question: when I read a sample (with CellQuest on a Mac G3) in the count event window there are too many events in the first seconds (700-800), then after 4 or 5 sec the number of events becomes omogenous (200-300). Someone (a technic from Becton Dickinson) said me that actually when the instrument is in the run mode it is acquiring from the sample and then, when I start manually from the palette, simply the CellQuest program connects to the FacsCalibur and it starts recording the data. I think that there is a lag time in this connection so that the CellQuest program is not able to show me the events in the first seconds from the start, so it sums the lacking data to the first data shown in the event count data window. Is it that correct? Thanks in advance Samuele Secchiero Department of Physiology and Pathology, University of Trieste Italy ___________________________________ Nuovo Yahoo! Messenger: E' molto pił divertente: Audibles, Avatar, Webcam, Giochi, Rubrica… Scaricalo ora! http://it.messenger.yahoo.itReceived on Wed Nov 17 18:58:00 2004
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