RE: ELISA vs CBA

We found that cytokine ELISA and bead arrays (for Luminex) were comparable
if kits from the same vendor were compared. Comparisons between vendors
yielded many more differences. We think this is likely due to the different
avidity of the various antibodies used. See Khan et al Cytometry 61B:35-39,
2004 for more details.

Phil

> -----Original Message-----
> From: Ruth Wilkins [mailto:Ruth_Wilkins@hc-sc.gc.ca] 
> Sent: Friday, November 12, 2004 11:31 AM
> To: Cytometry Mailing List
> Subject: ELISA vs CBA
> 
> 
> We have recently run a comparison between ELISA and CBA.  We 
> used the human inflammatory CBA kit and ran a few samples 
> that we had previously run on an IL-6 ELISA.	We were 
> surprised to find that the CBA results were about twice the 
> ELISA results.  Has anyone else had this experience?
> 
> Thanks,
> 
> ____________________
> Ruth Wilkins, PhD
> Research Scientist
> Consumer and Clinical Radiation Protection Bureau
> Health Canada
> 775 Brookfield Road, PL 6303B
> Ottawa, ON  K1A 1C1
> (613) 941-7263
> FAX (613) 941-1734
> 
> 
>